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- W2058427054 abstract "THE prereplicative viral messenger RNA that is made in bacteriophage T4 infected E. coli during the first 5 min of infection (at 30° C) hybridizes almost exclusively with the l strand of its DNA1,2. This strand selection results solely from the interaction of the bacterial RNA synthesizing machinery and the infecting phage DNA; at least it does not require any protein programmed by the virus, for it takes place even when the infection is carried out in the presence of chloramphenicol. At later times of the normal viral development, several new RNA species, some of which are transcribed off the viral r strand, appear (refs. 1, 2 and results of A. G. W. Salser, Bolle, W. Epstein, Pulitzer, E. P. G. and W. S., to be published). In this communication we report that the in vitro transcription of the mature T4 viral DNA template by purified E. coli RNA polymerase is restricted almost absolutely to the l strand of the DNA." @default.
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- W2058427054 date "1969-12-01" @default.
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- W2058427054 title "Transcription of the Bacteriophage T4 Template: Strand Selection by E. coli RNA Polymerase in vitro" @default.
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- W2058427054 doi "https://doi.org/10.1038/2241105a0" @default.
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