FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2058440062> ?p ?o ?g. }

• W2058440062 endingPage "1790" @default.
• W2058440062 startingPage "1785" @default.
• W2058440062 abstract "The recombinant N-acyl-d-amino acid amidohydrolase (N-d-AAase) of Variovorax paradoxus Iso1 was unstable during protein purification and storage at 4 °C. Since the methionine oxidation might be the artificial factor leading to the inactivation of N-d-AAase, eight potential oxidation sensitive methionine residues of the enzyme were individually substituted with leucine utilizing site-directed mutagenesis. Among them, five mutants, M39L, M56L, M221L, M254L, and M352L remained at least 70% of wild-type specific activity. The enzyme kinetic parameters of M221L revealed a 44% decrease in Km, and finally reflected a 2.4-fold increase in kcat/Km. Moreover, its half-life at 4 °C increased up to 6-fold longer than that of the wild-type. Structural analysis of each methionine substitution was carried out based on the crystal structure of N-d-AAase from Alcaligenes faecalis DA1. Met221 spatial closeness to the zinc-assistant catalytic center is highly potential as the primary site for oxidative inactivation. We conclude that the replacement of methionine M221 with leucine in N-d-AAase successfully enhances the oxidative resistance, half-life, and enzyme activity. This finding provides a promising basis for the engineering the stability and activity of N-d-AAase." @default.
• W2058440062 created "2016-06-24" @default.
• W2058440062 creator A5028936522 @default.
• W2058440062 creator A5052055095 @default.
• W2058440062 creator A5060245538 @default.
• W2058440062 creator A5071674838 @default.
• W2058440062 date "2012-12-01" @default.
• W2058440062 modified "2023-09-26" @default.
• W2058440062 title "Increasing the storage and oxidation stabilities of N-acyl-d-amino acid amidohydrolase by site-directed mutagenesis of critical methionine residues" @default.
• W2058440062 cites W1511290628 @default.
• W2058440062 cites W1540202700 @default.
• W2058440062 cites W1551576343 @default.
• W2058440062 cites W1590376353 @default.
• W2058440062 cites W1591436639 @default.
• W2058440062 cites W1949047900 @default.
• W2058440062 cites W1967523537 @default.
• W2058440062 cites W1977797772 @default.
• W2058440062 cites W1977871129 @default.
• W2058440062 cites W1980518713 @default.
• W2058440062 cites W1997678546 @default.
• W2058440062 cites W2001687138 @default.
• W2058440062 cites W2010096286 @default.
• W2058440062 cites W2020025535 @default.
• W2058440062 cites W2026199113 @default.
• W2058440062 cites W2041188821 @default.
• W2058440062 cites W2042577847 @default.
• W2058440062 cites W2044205462 @default.
• W2058440062 cites W2058089145 @default.
• W2058440062 cites W2060809301 @default.
• W2058440062 cites W2065574400 @default.
• W2058440062 cites W2071707850 @default.
• W2058440062 cites W2088088066 @default.
• W2058440062 cites W2090299387 @default.
• W2058440062 cites W2093929400 @default.
• W2058440062 cites W2100837269 @default.
• W2058440062 cites W2123537837 @default.
• W2058440062 cites W2124892883 @default.
• W2058440062 cites W2130920644 @default.
• W2058440062 cites W2138270253 @default.
• W2058440062 cites W2155925913 @default.
• W2058440062 cites W2177092266 @default.
• W2058440062 cites W3207586186 @default.
• W2058440062 cites W4293247451 @default.
• W2058440062 doi "https://doi.org/10.1016/j.procbio.2012.06.003" @default.
• W2058440062 hasPublicationYear "2012" @default.
• W2058440062 type Work @default.
• W2058440062 sameAs 2058440062 @default.
• W2058440062 citedByCount "7" @default.
• W2058440062 countsByYear W20584400622013 @default.
• W2058440062 countsByYear W20584400622015 @default.
• W2058440062 countsByYear W20584400622016 @default.
• W2058440062 countsByYear W20584400622021 @default.
• W2058440062 countsByYear W20584400622022 @default.
• W2058440062 countsByYear W20584400622023 @default.
• W2058440062 crossrefType "journal-article" @default.
• W2058440062 hasAuthorship W2058440062A5028936522 @default.
• W2058440062 hasAuthorship W2058440062A5052055095 @default.
• W2058440062 hasAuthorship W2058440062A5060245538 @default.
• W2058440062 hasAuthorship W2058440062A5071674838 @default.
• W2058440062 hasBestOaLocation W20584400622 @default.
• W2058440062 hasConcept C103408237 @default.
• W2058440062 hasConcept C104317684 @default.
• W2058440062 hasConcept C143065580 @default.
• W2058440062 hasConcept C16318435 @default.
• W2058440062 hasConcept C181199279 @default.
• W2058440062 hasConcept C185592680 @default.
• W2058440062 hasConcept C2779201268 @default.
• W2058440062 hasConcept C2779485144 @default.
• W2058440062 hasConcept C2780912031 @default.
• W2058440062 hasConcept C41183919 @default.
• W2058440062 hasConcept C515207424 @default.
• W2058440062 hasConcept C55493867 @default.
• W2058440062 hasConcept C56856141 @default.
• W2058440062 hasConcept C71240020 @default.
• W2058440062 hasConceptScore W2058440062C103408237 @default.
• W2058440062 hasConceptScore W2058440062C104317684 @default.
• W2058440062 hasConceptScore W2058440062C143065580 @default.
• W2058440062 hasConceptScore W2058440062C16318435 @default.
• W2058440062 hasConceptScore W2058440062C181199279 @default.
• W2058440062 hasConceptScore W2058440062C185592680 @default.
• W2058440062 hasConceptScore W2058440062C2779201268 @default.
• W2058440062 hasConceptScore W2058440062C2779485144 @default.
• W2058440062 hasConceptScore W2058440062C2780912031 @default.
• W2058440062 hasConceptScore W2058440062C41183919 @default.
• W2058440062 hasConceptScore W2058440062C515207424 @default.
• W2058440062 hasConceptScore W2058440062C55493867 @default.
• W2058440062 hasConceptScore W2058440062C56856141 @default.
• W2058440062 hasConceptScore W2058440062C71240020 @default.
• W2058440062 hasIssue "12" @default.
• W2058440062 hasLocation W20584400621 @default.
• W2058440062 hasLocation W20584400622 @default.
• W2058440062 hasOpenAccess W2058440062 @default.
• W2058440062 hasPrimaryLocation W20584400621 @default.
• W2058440062 hasRelatedWork W2013959643 @default.
• W2058440062 hasRelatedWork W2017218857 @default.
• W2058440062 hasRelatedWork W2047434710 @default.
• W2058440062 hasRelatedWork W2053583806 @default.
• W2058440062 hasRelatedWork W2058440062 @default.

• next