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- W2058462913 abstract "The construction of a multiplex system for recording the resonance Raman (RR) spectra of enzyme-substrate complexes in the near-UV region is described. The instrument is based on commercially available components consisting of a 2 W UV krypton ion laser, a triple spectrograph (in which the first two monochromators act as a zero dispersion, variable bandpass filter) and an intensified diode array. The performance of this system is compared with that of a double monochromator with a photomultiplier as a detector. The enzyme-substrate systems chosen as examples for the comparison offer a difficult experimental challenge because they evolve rapidly and have poor optical homogeneity. In addition, a simple apparatus is described for recording the RR spectra of enzyme-substrate complexes in cryosolvents at temperatures as low as −50 °C." @default.
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- W2058462913 date "1983-08-01" @default.
- W2058462913 modified "2023-09-25" @default.
- W2058462913 title "Diode array versus photomultiplier for measuring the UV-excited resonance Raman spectra of enzyme-substrate transients" @default.
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- W2058462913 doi "https://doi.org/10.1002/jrs.1250140412" @default.
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