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- W2058497997 abstract "This chapter explores some of the pitfalls associated with actin purification and to clarify in some detail the correct use and expected result from each step of the widely used muscle actin purification procedure. Typical preparations use about 10 g of acetone powder. The steps include in the purification are following: extraction of acetone powder; filtration using sterile cheesecloth; centrifugation; polymerization; high salt wash: tropomyosin removal; sedimentation of filamentous actin; depolymerization. It is found that extensive dialysis results in eventual dissociation of actin-myosin complexes and appearance of myosin in the final product. Prolonged dialysis can also lead to observable actin proteolysis and should therefore be avoided. Final steps are Clarification of G-actinadn Polymerization. Depending upon individual technique, buffer purity, dialysis times employed, and so forth, the actin preparation can contain small amounts of contaminating protein, including proteolysed actin. Thus, the chapter presents several techniques for further purification of the actin." @default.
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- W2058497997 date "1982-01-01" @default.
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- W2058497997 title "[18] Purification of muscle actin" @default.
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- W2058497997 doi "https://doi.org/10.1016/0076-6879(82)85020-9" @default.
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