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• W2058498191 abstract "Abstract Forward genetic screens provide a powerful approach for inferring gene function on the basis of the phenotypes associated with mutated genes. However, determining the causal mutation by traditional mapping and candidate gene sequencing is often the rate-limiting step, especially when analyzing many mutants. We report two genomic approaches for more rapidly determining the identity of the affected genes in Caenorhabditis elegans mutants. First, we report our use of restriction site-associated DNA (RAD) polymorphism markers for rapidly mapping mutations after chemical mutagenesis and mutant isolation. Second, we describe our use of genomic interval pull-down sequencing (GIPS) to selectively capture and sequence megabase-sized portions of a mutant genome. Together, these two methods provide a rapid and cost-effective approach for positional cloning of C. elegans mutant loci, and are also applicable to other genetic model systems." @default.
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• W2058498191 date "2011-11-01" @default.
• W2058498191 modified "2023-10-06" @default.
• W2058498191 title "Rapid Mapping and Identification of Mutations in<i>Caenorhabditis elegans</i>by Restriction Site-Associated DNA Mapping and Genomic Interval Pull-Down Sequencing" @default.
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• W2058498191 doi "https://doi.org/10.1534/genetics.111.134031" @default.
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