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• W2058500536 abstract "Standard methods for contrasting ultrathin sections generally have their greatest effect on cells and cellular components, whereas extracellular elements remain relatively electron-lucent. Occasionally, some extracellular elements even fail completely to react with the staining solutions. We describe a method for rendering a uniformly high contrast to extracellular tissue components. This consists of a brief prestaining of grids with diluted tannic acid in distilled water. Simple, rapid, and versatile, this procedure can be routinely applied to all tissue samples examined by electron microscopy. As an additional advantage, the method greatly enhances the electron density of intracellular glycogen. Higher concentrations of tannic acid give increased electron density, especially to elastin, and can therefore be used as an elastin stain." @default.
• W2058500536 created "2016-06-24" @default.
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• W2058500536 date "1990-01-01" @default.
• W2058500536 modified "2023-10-16" @default.
• W2058500536 title "Rapid Contrasting of Extracellular Elements in Thin Sections" @default.
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• W2058500536 doi "https://doi.org/10.3109/01913129009076139" @default.
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