FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2058520242> ?p ?o ?g. }

• W2058520242 endingPage "e1358" @default.
• W2058520242 startingPage "e1358" @default.
• W2058520242 abstract "BH3 interacting-domain death agonist (Bid) is a BH3-only pro-apoptotic member of the Bcl-2 family of proteins. Its function in apoptosis is associated with the proteolytic cleavage to the truncated form tBid, mainly by caspase-8. tBid translocates to mitochondria and assists Bax and Bak in induction of apoptosis. c-Jun N-terminal kinase (JNK)-dependent alternative processing of Bid to jBid was also reported. We have previously shown that the folate stress enzyme 10-formyltetrahydrofolate dehydrogenase (ALDH1L1) activates JNK1 and JNK2 in cancer cells as a pro-apoptotic response. Here we report that in PC-3 prostate cancer cells, JNK1/2 phosphorylate Bid at Thr59 within the caspase cleavage site in response to ALDH1L1. In vitro, all three JNK isoforms, JNK 1-3, phosphorylated Thr59 of Bid with JNK1 being the least active. Thr59 phosphorylation protected Bid from cleavage by caspase-8, resulting in strong accumulation of the full-length protein and its translocation to mitochondria. Interestingly, although we did not observe jBid in response to ALDH1L1 in PC-3 cells, transient expression of Bid mutants lacking the caspase-8 cleavage site resulted in strong accumulation of jBid. Of note, a T59D mutant mimicking constitutive phosphorylation revealed more profound cleavage of Bid to jBid. JNK-driven Bid accumulation had a pro-apoptotic effect in our study: small interfering RNA silencing of either JNK1/2 or Bid prevented Bid phosphorylation and accumulation, and rescued ALDH1L1-expressing cells. As full-length Bid is a weaker apoptogen than tBid, we propose that the phosphorylation of Bid by JNKs, followed by the accumulation of the full-length protein, delays attainment of apoptosis, and allows the cell to evaluate the stress and make a decision regarding the response strategy. This mechanism perhaps can be modified by the alternative cleavage of phospho-T59 Bid to jBid at some conditions." @default.
• W2058520242 created "2016-06-24" @default.
• W2058520242 creator A5037018298 @default.
• W2058520242 creator A5045024571 @default.
• W2058520242 creator A5053486647 @default.
• W2058520242 creator A5067376130 @default.
• W2058520242 creator A5074525368 @default.
• W2058520242 creator A5079214922 @default.
• W2058520242 creator A5083752966 @default.
• W2058520242 date "2014-07-31" @default.
• W2058520242 modified "2023-10-18" @default.
• W2058520242 title "JNK1/2 regulate Bid by direct phosphorylation at Thr59 in response to ALDH1L1" @default.
• W2058520242 cites W1522624066 @default.
• W2058520242 cites W1562623205 @default.
• W2058520242 cites W1823288226 @default.
• W2058520242 cites W1965078913 @default.
• W2058520242 cites W1965512256 @default.
• W2058520242 cites W1966073463 @default.
• W2058520242 cites W1966291495 @default.
• W2058520242 cites W1971503768 @default.
• W2058520242 cites W1976519846 @default.
• W2058520242 cites W1984547206 @default.
• W2058520242 cites W1985951335 @default.
• W2058520242 cites W1992425314 @default.
• W2058520242 cites W1993351141 @default.
• W2058520242 cites W1996463118 @default.
• W2058520242 cites W2000834350 @default.
• W2058520242 cites W2001543531 @default.
• W2058520242 cites W2009776832 @default.
• W2058520242 cites W2012173926 @default.
• W2058520242 cites W2012324453 @default.
• W2058520242 cites W2013658872 @default.
• W2058520242 cites W2015157788 @default.
• W2058520242 cites W2018692481 @default.
• W2058520242 cites W2028134112 @default.
• W2058520242 cites W2029797799 @default.
• W2058520242 cites W2031336272 @default.
• W2058520242 cites W2033181253 @default.
• W2058520242 cites W2038100199 @default.
• W2058520242 cites W2045823608 @default.
• W2058520242 cites W2055262533 @default.
• W2058520242 cites W2057292793 @default.
• W2058520242 cites W2062853648 @default.
• W2058520242 cites W2066787736 @default.
• W2058520242 cites W2073028327 @default.
• W2058520242 cites W2074013757 @default.
• W2058520242 cites W2080389159 @default.
• W2058520242 cites W2084499744 @default.
• W2058520242 cites W2087615058 @default.
• W2058520242 cites W2090970397 @default.
• W2058520242 cites W2094433211 @default.
• W2058520242 cites W2097672870 @default.
• W2058520242 cites W2098609440 @default.
• W2058520242 cites W2103381809 @default.
• W2058520242 cites W2105253445 @default.
• W2058520242 cites W2109017691 @default.
• W2058520242 cites W2109508815 @default.
• W2058520242 cites W2109956888 @default.
• W2058520242 cites W2127364281 @default.
• W2058520242 cites W2134461910 @default.
• W2058520242 cites W2136269288 @default.
• W2058520242 cites W2142120946 @default.
• W2058520242 cites W2145411963 @default.
• W2058520242 cites W2148061274 @default.
• W2058520242 cites W2149789119 @default.
• W2058520242 cites W2150315067 @default.
• W2058520242 cites W2152041471 @default.
• W2058520242 cites W2152247516 @default.
• W2058520242 cites W2160169717 @default.
• W2058520242 cites W2162560719 @default.
• W2058520242 cites W2168726708 @default.
• W2058520242 cites W2325862576 @default.
• W2058520242 doi "https://doi.org/10.1038/cddis.2014.316" @default.
• W2058520242 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4123105" @default.
• W2058520242 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25077544" @default.
• W2058520242 hasPublicationYear "2014" @default.
• W2058520242 type Work @default.
• W2058520242 sameAs 2058520242 @default.
• W2058520242 citedByCount "19" @default.
• W2058520242 countsByYear W20585202422015 @default.
• W2058520242 countsByYear W20585202422016 @default.
• W2058520242 countsByYear W20585202422017 @default.
• W2058520242 countsByYear W20585202422018 @default.
• W2058520242 countsByYear W20585202422019 @default.
• W2058520242 countsByYear W20585202422020 @default.
• W2058520242 countsByYear W20585202422021 @default.
• W2058520242 countsByYear W20585202422022 @default.
• W2058520242 countsByYear W20585202422023 @default.
• W2058520242 crossrefType "journal-article" @default.
• W2058520242 hasAuthorship W2058520242A5037018298 @default.
• W2058520242 hasAuthorship W2058520242A5045024571 @default.
• W2058520242 hasAuthorship W2058520242A5053486647 @default.
• W2058520242 hasAuthorship W2058520242A5067376130 @default.
• W2058520242 hasAuthorship W2058520242A5074525368 @default.
• W2058520242 hasAuthorship W2058520242A5079214922 @default.
• W2058520242 hasAuthorship W2058520242A5083752966 @default.
• W2058520242 hasBestOaLocation W20585202421 @default.
• W2058520242 hasConcept C104317684 @default.

• next