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- W2058553674 abstract "Abstract We report on the development of a detailed two‐dimensional electrophoresis map of Chinese hamster ovary (CHO) cell proteins based on fluorescence staining and tandem time‐of‐flight (TOF/TOF)‐mass spectrometry. We observed a 71% success rate in the identification of proteins even though the CHO genome is not sequenced. The map consists of 224 protein identifications present in 274 two‐dimensional gel electrophoresis (2‐DE) gel spots. We have also initiated a study of the phosphoproteome using a commercially available phosphoprotein‐specific fluorescent stain. Using this stain, we observe 672 phosphorylated proteins, including many proteins known to be phosphorylated, which is 36% of the proteins we visualized with a total protein stain and consistent with expectations." @default.
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- W2058553674 date "2004-08-01" @default.
- W2058553674 modified "2023-10-17" @default.
- W2058553674 title "A two‐dimensional electrophoresis map of Chinese hamster ovary cell proteins based on fluorescence staining" @default.
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- W2058553674 doi "https://doi.org/10.1002/elps.200406010" @default.
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