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- W2058589044 abstract "An improved kinetic rate-determination for measuring the activity of isocitrate dehydrogenase (EC 1.1.1.42; D8-isocitrate: NADP oxidoreductase (decarboxylating); ICDH) by monitoring the increased absorbance at 340 nm consequent upon reduction of NADP is described. The method employs higher NADP concentration (0.42 mM) than has previously been used (0.127 mM) and the use of NaCl, shown to be inhibitory, is avoided. Some kinetic properties of the enzyme in human liver supernatan, and sera from patients with infectious hepatitis, have been compared. The serum enzyme has a significantly higher Km-NADP (p < 0.001) and unlike the liver enzyme is not inhibited by magnesium concentrations between 17 and 75 mM. Despite problems with a serum blank and a lag after substrate injection, the estimation can be successfully adapted for routine semi-automatic use with the aid of a Reaction Rate Analyser. The within-batch precision obtained with several samples was satisfactory, the Coefficient of Variation ranging from 1.2% to 3.5%. The factors contributing to the blank reaction are described. It seldom exceeds a rate equivalent to 1 IU/litre, and when ignored, the normal range for serum ICDH at 37° obtained through study of three different populations was 0–7 IU/litre." @default.
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- W2058589044 title "An improved manual and semi-automatic assay for NADP-dependent isocitrate dehydrogenase activity, with a description of some kinetic properties of human liver and serum enzyme" @default.
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- W2058589044 doi "https://doi.org/10.1016/s0009-9120(71)91363-4" @default.
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