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- W2058602004 abstract "A DNA fragment coding for the E. coli phoA signal peptide was synthesized and inserted into the expression vector pKK223‐3. A single Hin dIII restriction site is located just at the end of the signal sequence. A gene coding for the proteinase inhibitor hirudin, which has previously been synthesized, was inserted into this Hin dIII site. The hybrid protein was expressed under control of the tac‐promoter and secreted into the periplasm of E. coli . From the periplasmic fraction two processed proteins were isolated. One of these was identical with desulfatohirudin and also had similar biological properties." @default.
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- W2058602004 date "1986-07-07" @default.
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- W2058602004 title "Expression, secretion and processing of hirudin in <i>E. coli</i> using the alkaline phosphatase signal sequence" @default.
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- W2058602004 doi "https://doi.org/10.1016/0014-5793(86)80721-9" @default.
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