Matches in SemOpenAlex for { <https://semopenalex.org/work/W2058660431> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W2058660431 endingPage "227" @default.
- W2058660431 startingPage "220" @default.
- W2058660431 abstract "Clostridium botulinum type G cultured for 6 days at 30 degrees C in proteose peptone-yeast extract-glucose medium produced toxin of 1.3 x 10(4) LD50/ml. The toxin was precipitated at pH 4.0, extracted with 0.2 M phosphate buffer, pH 6.0, and activated with trypsin. Sonic treatment and trypsinization of the residual precipitate released additional toxin, the toxicity of which corresponded to that detected in whole culture. Activated toxin obtained from the first extract and that from the residual precipitate were combined and purified by salting out, acid precipitation, gel filtration on Sephadex G-200, chromatography on SP-Sephadex, and a second gel filtration on Sephadex G-200. The yield of purified toxin from 10 liters of culture was 22.9 mg an 1.1 X 10(8) mouse ip LD50 with a specific toxicity of 3.0 X 10(7) mouse ip LD50/mg nitrogen. The molecular weight of the toxin was about 500,000, corresponding to that of L toxin of the other types. No M nor LL toxin was detected." @default.
- W2058660431 created "2016-06-24" @default.
- W2058660431 creator A5003799538 @default.
- W2058660431 creator A5045155543 @default.
- W2058660431 creator A5060625803 @default.
- W2058660431 creator A5079321807 @default.
- W2058660431 date "1988-04-01" @default.
- W2058660431 modified "2023-09-27" @default.
- W2058660431 title "Purification of Clostridium botulinum type G progenitor toxin" @default.
- W2058660431 cites W1494680985 @default.
- W2058660431 cites W1603297792 @default.
- W2058660431 cites W1775749144 @default.
- W2058660431 cites W2041408554 @default.
- W2058660431 cites W2050335943 @default.
- W2058660431 cites W2067938759 @default.
- W2058660431 cites W2082708477 @default.
- W2058660431 cites W2094451620 @default.
- W2058660431 cites W2137302959 @default.
- W2058660431 cites W2557808926 @default.
- W2058660431 doi "https://doi.org/10.1016/s0176-6724(88)80006-3" @default.
- W2058660431 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3293334" @default.
- W2058660431 hasPublicationYear "1988" @default.
- W2058660431 type Work @default.
- W2058660431 sameAs 2058660431 @default.
- W2058660431 citedByCount "4" @default.
- W2058660431 countsByYear W20586604312017 @default.
- W2058660431 countsByYear W20586604312022 @default.
- W2058660431 crossrefType "journal-article" @default.
- W2058660431 hasAuthorship W2058660431A5003799538 @default.
- W2058660431 hasAuthorship W2058660431A5045155543 @default.
- W2058660431 hasAuthorship W2058660431A5060625803 @default.
- W2058660431 hasAuthorship W2058660431A5079321807 @default.
- W2058660431 hasConcept C163994747 @default.
- W2058660431 hasConcept C178790620 @default.
- W2058660431 hasConcept C181199279 @default.
- W2058660431 hasConcept C185592680 @default.
- W2058660431 hasConcept C193363816 @default.
- W2058660431 hasConcept C2777010668 @default.
- W2058660431 hasConcept C2777367657 @default.
- W2058660431 hasConcept C2780340462 @default.
- W2058660431 hasConcept C29730261 @default.
- W2058660431 hasConcept C38240268 @default.
- W2058660431 hasConcept C43617362 @default.
- W2058660431 hasConcept C55493867 @default.
- W2058660431 hasConcept C86803240 @default.
- W2058660431 hasConcept C89423630 @default.
- W2058660431 hasConceptScore W2058660431C163994747 @default.
- W2058660431 hasConceptScore W2058660431C178790620 @default.
- W2058660431 hasConceptScore W2058660431C181199279 @default.
- W2058660431 hasConceptScore W2058660431C185592680 @default.
- W2058660431 hasConceptScore W2058660431C193363816 @default.
- W2058660431 hasConceptScore W2058660431C2777010668 @default.
- W2058660431 hasConceptScore W2058660431C2777367657 @default.
- W2058660431 hasConceptScore W2058660431C2780340462 @default.
- W2058660431 hasConceptScore W2058660431C29730261 @default.
- W2058660431 hasConceptScore W2058660431C38240268 @default.
- W2058660431 hasConceptScore W2058660431C43617362 @default.
- W2058660431 hasConceptScore W2058660431C55493867 @default.
- W2058660431 hasConceptScore W2058660431C86803240 @default.
- W2058660431 hasConceptScore W2058660431C89423630 @default.
- W2058660431 hasIssue "2" @default.
- W2058660431 hasLocation W20586604311 @default.
- W2058660431 hasLocation W20586604312 @default.
- W2058660431 hasOpenAccess W2058660431 @default.
- W2058660431 hasPrimaryLocation W20586604311 @default.
- W2058660431 hasRelatedWork W1488152201 @default.
- W2058660431 hasRelatedWork W1499343043 @default.
- W2058660431 hasRelatedWork W1916885135 @default.
- W2058660431 hasRelatedWork W1942618335 @default.
- W2058660431 hasRelatedWork W1943620681 @default.
- W2058660431 hasRelatedWork W1975880046 @default.
- W2058660431 hasRelatedWork W1980423755 @default.
- W2058660431 hasRelatedWork W2058660431 @default.
- W2058660431 hasRelatedWork W2418070691 @default.
- W2058660431 hasRelatedWork W4235511038 @default.
- W2058660431 hasVolume "268" @default.
- W2058660431 isParatext "false" @default.
- W2058660431 isRetracted "false" @default.
- W2058660431 magId "2058660431" @default.
- W2058660431 workType "article" @default.