Matches in SemOpenAlex for { <https://semopenalex.org/work/W2058670203> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2058670203 endingPage "1430" @default.
- W2058670203 startingPage "1421" @default.
- W2058670203 abstract "Tolerance to various stresses is a key phenotype for cell catalysts, which are used widely in bioproduction of diverse valuable chemicals. Using the Rhodococcus ruber TH strain, which exhibits high nitrile hydratase activity, as the target cell catalyst for acrylamide production, we established a method to improve cell tolerance by stably introducing global transcription perturbation. The σ(70) gene (sigA) of R. ruber was cloned and randomly mutated. An R. ruber TH3/pNV-sigA(M) library containing additional sigA mutants was constructed and used for survival selection. The TH3/M4N1-59 mutant was selected by acrylonitrile/acrylamide double stress and exhibited a 160 % extension of the half-life of nitrile hydratase upon exposure to 40 % acrylamide. A redesigned parDE(M) gene was introduced to Rhodococcus to accomplish stable inheritance of plasmids. A two-batch acrylonitrile hydration reaction was performed using the engineered cells as a catalyst. Compared to TH3, the acrylamide productivity of TH3/M4N1-59DE(M) catalysis increased by 27.8 and 37.5 % in the first and second bioreaction batches, respectively. These data suggest a novel method for increasing the bioconversion productivity of target chemicals through sigA mutation of the cell catalyst." @default.
- W2058670203 created "2016-06-24" @default.
- W2058670203 creator A5048299423 @default.
- W2058670203 creator A5077632892 @default.
- W2058670203 date "2012-10-01" @default.
- W2058670203 modified "2023-10-16" @default.
- W2058670203 title "Engineering of<i> Rhodococcus</i> cell catalysts for tolerance improvement by sigma factor mutation and active plasmid partition" @default.
- W2058670203 cites W1936065549 @default.
- W2058670203 cites W1957843310 @default.
- W2058670203 cites W1975381809 @default.
- W2058670203 cites W2006313129 @default.
- W2058670203 cites W2006552859 @default.
- W2058670203 cites W2017116917 @default.
- W2058670203 cites W2023686356 @default.
- W2058670203 cites W2024914551 @default.
- W2058670203 cites W2053810265 @default.
- W2058670203 cites W2067482629 @default.
- W2058670203 cites W2080537888 @default.
- W2058670203 cites W2109918485 @default.
- W2058670203 cites W2122093608 @default.
- W2058670203 cites W2142094178 @default.
- W2058670203 cites W2144653683 @default.
- W2058670203 cites W2328504023 @default.
- W2058670203 cites W2334049226 @default.
- W2058670203 doi "https://doi.org/10.1007/s10295-012-1146-5" @default.
- W2058670203 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22638790" @default.
- W2058670203 hasPublicationYear "2012" @default.
- W2058670203 type Work @default.
- W2058670203 sameAs 2058670203 @default.
- W2058670203 citedByCount "22" @default.
- W2058670203 countsByYear W20586702032013 @default.
- W2058670203 countsByYear W20586702032014 @default.
- W2058670203 countsByYear W20586702032015 @default.
- W2058670203 countsByYear W20586702032016 @default.
- W2058670203 countsByYear W20586702032017 @default.
- W2058670203 countsByYear W20586702032018 @default.
- W2058670203 countsByYear W20586702032019 @default.
- W2058670203 countsByYear W20586702032020 @default.
- W2058670203 countsByYear W20586702032021 @default.
- W2058670203 countsByYear W20586702032022 @default.
- W2058670203 crossrefType "journal-article" @default.
- W2058670203 hasAuthorship W2058670203A5048299423 @default.
- W2058670203 hasAuthorship W2058670203A5077632892 @default.
- W2058670203 hasConcept C104317684 @default.
- W2058670203 hasConcept C153911025 @default.
- W2058670203 hasConcept C15920480 @default.
- W2058670203 hasConcept C161790260 @default.
- W2058670203 hasConcept C166940927 @default.
- W2058670203 hasConcept C178790620 @default.
- W2058670203 hasConcept C181199279 @default.
- W2058670203 hasConcept C185592680 @default.
- W2058670203 hasConcept C22744801 @default.
- W2058670203 hasConcept C2778372188 @default.
- W2058670203 hasConcept C2779122190 @default.
- W2058670203 hasConcept C2779227428 @default.
- W2058670203 hasConcept C2779402116 @default.
- W2058670203 hasConcept C2779952263 @default.
- W2058670203 hasConcept C521977710 @default.
- W2058670203 hasConcept C55493867 @default.
- W2058670203 hasConcept C86803240 @default.
- W2058670203 hasConceptScore W2058670203C104317684 @default.
- W2058670203 hasConceptScore W2058670203C153911025 @default.
- W2058670203 hasConceptScore W2058670203C15920480 @default.
- W2058670203 hasConceptScore W2058670203C161790260 @default.
- W2058670203 hasConceptScore W2058670203C166940927 @default.
- W2058670203 hasConceptScore W2058670203C178790620 @default.
- W2058670203 hasConceptScore W2058670203C181199279 @default.
- W2058670203 hasConceptScore W2058670203C185592680 @default.
- W2058670203 hasConceptScore W2058670203C22744801 @default.
- W2058670203 hasConceptScore W2058670203C2778372188 @default.
- W2058670203 hasConceptScore W2058670203C2779122190 @default.
- W2058670203 hasConceptScore W2058670203C2779227428 @default.
- W2058670203 hasConceptScore W2058670203C2779402116 @default.
- W2058670203 hasConceptScore W2058670203C2779952263 @default.
- W2058670203 hasConceptScore W2058670203C521977710 @default.
- W2058670203 hasConceptScore W2058670203C55493867 @default.
- W2058670203 hasConceptScore W2058670203C86803240 @default.
- W2058670203 hasIssue "10" @default.
- W2058670203 hasLocation W20586702031 @default.
- W2058670203 hasLocation W20586702032 @default.
- W2058670203 hasOpenAccess W2058670203 @default.
- W2058670203 hasPrimaryLocation W20586702031 @default.
- W2058670203 hasRelatedWork W1968143446 @default.
- W2058670203 hasRelatedWork W1978280518 @default.
- W2058670203 hasRelatedWork W1985901489 @default.
- W2058670203 hasRelatedWork W2058670203 @default.
- W2058670203 hasRelatedWork W2094496604 @default.
- W2058670203 hasRelatedWork W2314437412 @default.
- W2058670203 hasRelatedWork W2351284273 @default.
- W2058670203 hasRelatedWork W2381337866 @default.
- W2058670203 hasRelatedWork W2995183796 @default.
- W2058670203 hasRelatedWork W3108347267 @default.
- W2058670203 hasVolume "39" @default.
- W2058670203 isParatext "false" @default.
- W2058670203 isRetracted "false" @default.
- W2058670203 magId "2058670203" @default.
- W2058670203 workType "article" @default.