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• W2058679984 abstract "Conditions optimal for the transformation of Pseudomonas putida and E. coli with a drug-resistance factor (RP 1) DNA, which specifies resistance to carbenicillin, tetracycline, kanamycin, and neomycin, are described. The transformants retain all the fertility, incompatibility, and drug-resistance characteristics present in the parent. Covalently-closed circular molecules of almost identical contour lengths have been isolated from the parent and the transformants. The frequency of transformation is drastically reduced by treatment of RP 1 DNA with DNase and by denaturation or sonication. Shearing of RP 1 DNA in vitro and their subsequent introduction in P. putida cells, by transformation, produces transformants that exhibit a wide range of drug-resistant phenotypes, including those which are resistant to neomycin but sensitive to kanamycin. Isolation of such neomycin-resistant but kanamycin-sensitive transformants indicates that there might be two separate mechanisms specified by RP 1 for resistance to the two antibiotics." @default.
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• W2058679984 date "1975-09-01" @default.
• W2058679984 modified "2023-10-18" @default.
• W2058679984 title "Transformation of Pseudomonas putida and Escherichia coli with plasmid-linked drug-resistance factor DNA." @default.
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• W2058679984 doi "https://doi.org/10.1073/pnas.72.9.3647" @default.
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