Matches in SemOpenAlex for { <https://semopenalex.org/work/W2058715094> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2058715094 endingPage "99" @default.
- W2058715094 startingPage "93" @default.
- W2058715094 abstract "Human placental trophoblast invasion of the uterus is a highly controlled event. We had shown that transforming growth factor-beta (TGF-beta) produced in the pregnant uterus controls invasiveness and reduces proliferation of first trimester placental trophoblasts in vitro. The anti-invasive effect of TGF-beta was due, at least in part, to induction of tissue inhibitor of metalloproteinases (TIMP)-1. In the present study we compared the effects of TGF-beta on proliferation ([3H]-TdR incorporation) and invasiveness (3-day Matrigel invasion assay) of JAR and JEG-3 choriocarcinoma cells vs normal first trimester human trophoblast cells. Transcripts of type IV collagenases (72- and 92-kDa enzymes, i.e., gelatinases A and B) and their inhibitors (TIMP-1 and TIMP-2) in these cells were measured by Northern analysis, and secretion of gelatinases and plasminogen activators (PAs) was evaluated by gel zymography. The results revealed that: (a) TGF-beta inhibited invasiveness and proliferation of normal trophoblast but not JAR and JEG-3 choriocarcinoma cells; (b) gelatinase A mRNA, expressed by the normal trophoblast and JAR cells, was upregulated in the presence of TGF-beta; (c) gelatinase B mRNA was not detected in the total RNA preparations of treated or untreated normal trophoblast or choriocarcinoma cells; (d) TGF-beta significantly upregulated the levels of TIMP-1 mRNA in the normal trophoblasts, but this transcript was very low in treated as well as untreated choriocarcinoma cells; TGF-beta also upregulated the 3.5-kb TIMP-2 message in the normal trophoblast; (e) gelatin zymography revealed a distinct band of approximately 68-kDa (gelatinase A) in the conditioned media of normal trophoblast and JAR cells; however, TGF-beta did not change the level of secretion of this gelatinase; and (f) the normal trophoblast also exhibited significant PA secretion (casein zymography) which was reduced in the presence of TGF-beta. PA secretion by the malignant trophoblast cells was low and unaffected by TGF-beta. These findings suggest that choriocarcinoma cells may become refractory to the mechanisms which control normal trophoblast proliferation and invasiveness. Concurrent resistance to antiproliferative and anti-invasive molecules such as TGF-beta may be highly relevant to tumor progression." @default.
- W2058715094 created "2016-06-24" @default.
- W2058715094 creator A5010799165 @default.
- W2058715094 creator A5018171790 @default.
- W2058715094 creator A5059006575 @default.
- W2058715094 creator A5074231558 @default.
- W2058715094 creator A5086899527 @default.
- W2058715094 creator A5091380477 @default.
- W2058715094 date "1994-09-01" @default.
- W2058715094 modified "2023-10-16" @default.
- W2058715094 title "Resistance of Malignant Trophoblast Cells to both the Anti-proliferative and Anti-invasive Effects of Transforming Growth Factor-β" @default.
- W2058715094 doi "https://doi.org/10.1006/excr.1994.1237" @default.
- W2058715094 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8082752" @default.
- W2058715094 hasPublicationYear "1994" @default.
- W2058715094 type Work @default.
- W2058715094 sameAs 2058715094 @default.
- W2058715094 citedByCount "158" @default.
- W2058715094 countsByYear W20587150942012 @default.
- W2058715094 countsByYear W20587150942013 @default.
- W2058715094 countsByYear W20587150942014 @default.
- W2058715094 countsByYear W20587150942015 @default.
- W2058715094 countsByYear W20587150942016 @default.
- W2058715094 countsByYear W20587150942017 @default.
- W2058715094 countsByYear W20587150942018 @default.
- W2058715094 countsByYear W20587150942019 @default.
- W2058715094 countsByYear W20587150942020 @default.
- W2058715094 countsByYear W20587150942021 @default.
- W2058715094 countsByYear W20587150942022 @default.
- W2058715094 crossrefType "journal-article" @default.
- W2058715094 hasAuthorship W2058715094A5010799165 @default.
- W2058715094 hasAuthorship W2058715094A5018171790 @default.
- W2058715094 hasAuthorship W2058715094A5059006575 @default.
- W2058715094 hasAuthorship W2058715094A5074231558 @default.
- W2058715094 hasAuthorship W2058715094A5086899527 @default.
- W2058715094 hasAuthorship W2058715094A5091380477 @default.
- W2058715094 hasConcept C109523444 @default.
- W2058715094 hasConcept C118131993 @default.
- W2058715094 hasConcept C126322002 @default.
- W2058715094 hasConcept C134018914 @default.
- W2058715094 hasConcept C153911025 @default.
- W2058715094 hasConcept C16685009 @default.
- W2058715094 hasConcept C172680121 @default.
- W2058715094 hasConcept C2776232661 @default.
- W2058715094 hasConcept C2776953305 @default.
- W2058715094 hasConcept C2777495146 @default.
- W2058715094 hasConcept C2778229498 @default.
- W2058715094 hasConcept C2778608917 @default.
- W2058715094 hasConcept C2779234561 @default.
- W2058715094 hasConcept C2780832733 @default.
- W2058715094 hasConcept C2910246616 @default.
- W2058715094 hasConcept C54355233 @default.
- W2058715094 hasConcept C55493867 @default.
- W2058715094 hasConcept C71924100 @default.
- W2058715094 hasConcept C81885089 @default.
- W2058715094 hasConcept C86803240 @default.
- W2058715094 hasConceptScore W2058715094C109523444 @default.
- W2058715094 hasConceptScore W2058715094C118131993 @default.
- W2058715094 hasConceptScore W2058715094C126322002 @default.
- W2058715094 hasConceptScore W2058715094C134018914 @default.
- W2058715094 hasConceptScore W2058715094C153911025 @default.
- W2058715094 hasConceptScore W2058715094C16685009 @default.
- W2058715094 hasConceptScore W2058715094C172680121 @default.
- W2058715094 hasConceptScore W2058715094C2776232661 @default.
- W2058715094 hasConceptScore W2058715094C2776953305 @default.
- W2058715094 hasConceptScore W2058715094C2777495146 @default.
- W2058715094 hasConceptScore W2058715094C2778229498 @default.
- W2058715094 hasConceptScore W2058715094C2778608917 @default.
- W2058715094 hasConceptScore W2058715094C2779234561 @default.
- W2058715094 hasConceptScore W2058715094C2780832733 @default.
- W2058715094 hasConceptScore W2058715094C2910246616 @default.
- W2058715094 hasConceptScore W2058715094C54355233 @default.
- W2058715094 hasConceptScore W2058715094C55493867 @default.
- W2058715094 hasConceptScore W2058715094C71924100 @default.
- W2058715094 hasConceptScore W2058715094C81885089 @default.
- W2058715094 hasConceptScore W2058715094C86803240 @default.
- W2058715094 hasIssue "1" @default.
- W2058715094 hasLocation W20587150941 @default.
- W2058715094 hasLocation W20587150942 @default.
- W2058715094 hasOpenAccess W2058715094 @default.
- W2058715094 hasPrimaryLocation W20587150941 @default.
- W2058715094 hasRelatedWork W2003829652 @default.
- W2058715094 hasRelatedWork W2023444952 @default.
- W2058715094 hasRelatedWork W2035444091 @default.
- W2058715094 hasRelatedWork W2040877940 @default.
- W2058715094 hasRelatedWork W2058715094 @default.
- W2058715094 hasRelatedWork W2074953596 @default.
- W2058715094 hasRelatedWork W2089173686 @default.
- W2058715094 hasRelatedWork W2409992022 @default.
- W2058715094 hasRelatedWork W2479944675 @default.
- W2058715094 hasRelatedWork W3135202869 @default.
- W2058715094 hasVolume "214" @default.
- W2058715094 isParatext "false" @default.
- W2058715094 isRetracted "false" @default.
- W2058715094 magId "2058715094" @default.
- W2058715094 workType "article" @default.