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• W2058721844 abstract "Background: Current tissue-engineered cartilage constructs contain insufficient amounts of collagen, whose function is to resist tension. We postulate that dynamic tension is necessary to stimulate collagen formation. Another shortcoming is that tissue-engineered cartilage does not possess native zonal variations. We hypothesize that applying depth-varying mechanical cues would stimulate extracellular matrix (ECM) synthesis depth dependently. We developed a dedicated loading regime called sliding indentation, which enables us to apply dynamic tension as well as depth-varying strain fields to the chondrocyte-seeded agarose constructs. Objective: In 2 study designs, we explored whether sliding indentation would increase collagen content and induce depth-varying ECM distribution. Methods: In the first study, we developed an agarose-sandwich model that involves embedding of a thin chondrocyte-seeded 0.5% agarose layer between two cell-free 3% agarose layers. In the second study, 3-mm-thick chondrocyte-seeded agarose constructs were created. Sliding indentation at 10% depth and 1 Hz was applied to constructs in both studies for 4 h/day during 28 days, and unloaded constructs served as control. Results: Sliding indentation resulted in an increased amount of collagen in the produced cartilage layer. Further, sliding indentation for 7 days resulted in a depth-dependent response at gene expression levels, with the highest response in the regions that received highest strains. Analysis of protein expression after 28 days showed a similar depth-dependent distribution in all constructs, which further enhanced by sliding indentation. Conclusions: Sliding indentation can increase collagen content and enhances depth-dependent ECM distribution, and is therefore a promising strategy for culturing cartilage with improved properties." @default.
• W2058721844 created "2016-06-24" @default.
• W2058721844 creator A5018737322 @default.
• W2058721844 creator A5081456981 @default.
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• W2058721844 date "2013-09-01" @default.
• W2058721844 modified "2023-10-16" @default.
• W2058721844 title "Sliding Indentation Enhances Collagen Content and Depth-Dependent Matrix Distribution in Tissue-Engineered Cartilage Constructs" @default.
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• W2058721844 doi "https://doi.org/10.1089/ten.tea.2012.0688" @default.
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