Matches in SemOpenAlex for { <https://semopenalex.org/work/W2058746952> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2058746952 endingPage "430" @default.
- W2058746952 startingPage "426" @default.
- W2058746952 abstract "The ammonia-oxidizing chemoautotrophic Nitrosomonas sp. strain K1 exhibited marked ribulose-1,5-bisphosphate carboxylase (RubisCO) activity. The RubisCO [EC 4.1.1.39] was purified as an electrophoretically homogeneous protein. The molecular mass of the enzyme was estimated to be about 460 kDa by gel filtration, and it consists of two subunits [large (L): 52.2 kDa; small (S): 13.3 kDa] as demonstrated by SDS-PAGE. This confirmed that the enzyme has an L(8)S(8) structure. The K(m) values of the enzyme for RuBP, NaHCO3, and Mg2+ were estimated to be 0.112, 0.415, and 1.063 mM, respectively. The optimum pH and temperature for its activity were approximately 7.0 and 45 degrees C. The enzyme was stable up to 45 degrees C and in a pH range from 7.0-9.0 (4 degrees C, 48 h). The enzyme activity was inhibited by Cu2+, Hg2+, N-ethylmaleimide, p-chloromercuribenzoate, and SDS (0.1 mM). The activity was also inhibited by ammonium sulfate at high concentrations (38-303 mM) but the stability of the enzyme showed no inhibition at the same ammonium sulfate concentrations. The N-terminal amino acid sequences of the large and small subunits are AIKTYQAGVKEYRQTYW QPDYVPL and AIQAYHLTKKYETFSYLPQM, respectively." @default.
- W2058746952 created "2016-06-24" @default.
- W2058746952 creator A5001326276 @default.
- W2058746952 creator A5003669073 @default.
- W2058746952 creator A5006161186 @default.
- W2058746952 creator A5034444802 @default.
- W2058746952 creator A5044319604 @default.
- W2058746952 date "2000-01-01" @default.
- W2058746952 modified "2023-09-26" @default.
- W2058746952 title "Ribulose-1,5-bisphosphate carboxylase/oxygenase from an ammonia-oxidizing bacterium, Nitrosomonas sp. K1: Purification and properties" @default.
- W2058746952 cites W1497294959 @default.
- W2058746952 cites W1750773183 @default.
- W2058746952 cites W1982773572 @default.
- W2058746952 cites W1992403496 @default.
- W2058746952 cites W2019219042 @default.
- W2058746952 cites W2022961230 @default.
- W2058746952 cites W2035493569 @default.
- W2058746952 cites W2044019059 @default.
- W2058746952 cites W2054061426 @default.
- W2058746952 cites W2057319235 @default.
- W2058746952 cites W2084577127 @default.
- W2058746952 cites W2100837269 @default.
- W2058746952 cites W2103550580 @default.
- W2058746952 cites W2180256823 @default.
- W2058746952 cites W4233853615 @default.
- W2058746952 cites W4293247451 @default.
- W2058746952 doi "https://doi.org/10.1016/s1389-1723(01)80013-x" @default.
- W2058746952 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16232884" @default.
- W2058746952 hasPublicationYear "2000" @default.
- W2058746952 type Work @default.
- W2058746952 sameAs 2058746952 @default.
- W2058746952 citedByCount "10" @default.
- W2058746952 countsByYear W20587469522012 @default.
- W2058746952 countsByYear W20587469522013 @default.
- W2058746952 countsByYear W20587469522015 @default.
- W2058746952 countsByYear W20587469522022 @default.
- W2058746952 crossrefType "journal-article" @default.
- W2058746952 hasAuthorship W2058746952A5001326276 @default.
- W2058746952 hasAuthorship W2058746952A5003669073 @default.
- W2058746952 hasAuthorship W2058746952A5006161186 @default.
- W2058746952 hasAuthorship W2058746952A5034444802 @default.
- W2058746952 hasAuthorship W2058746952A5044319604 @default.
- W2058746952 hasConcept C140137476 @default.
- W2058746952 hasConcept C178790620 @default.
- W2058746952 hasConcept C181199279 @default.
- W2058746952 hasConcept C185592680 @default.
- W2058746952 hasConcept C193363816 @default.
- W2058746952 hasConcept C2776382133 @default.
- W2058746952 hasConcept C2777414806 @default.
- W2058746952 hasConcept C2779870022 @default.
- W2058746952 hasConcept C2779966244 @default.
- W2058746952 hasConcept C2780925461 @default.
- W2058746952 hasConcept C28212737 @default.
- W2058746952 hasConcept C43617362 @default.
- W2058746952 hasConcept C50212798 @default.
- W2058746952 hasConcept C55493867 @default.
- W2058746952 hasConcept C58121356 @default.
- W2058746952 hasConceptScore W2058746952C140137476 @default.
- W2058746952 hasConceptScore W2058746952C178790620 @default.
- W2058746952 hasConceptScore W2058746952C181199279 @default.
- W2058746952 hasConceptScore W2058746952C185592680 @default.
- W2058746952 hasConceptScore W2058746952C193363816 @default.
- W2058746952 hasConceptScore W2058746952C2776382133 @default.
- W2058746952 hasConceptScore W2058746952C2777414806 @default.
- W2058746952 hasConceptScore W2058746952C2779870022 @default.
- W2058746952 hasConceptScore W2058746952C2779966244 @default.
- W2058746952 hasConceptScore W2058746952C2780925461 @default.
- W2058746952 hasConceptScore W2058746952C28212737 @default.
- W2058746952 hasConceptScore W2058746952C43617362 @default.
- W2058746952 hasConceptScore W2058746952C50212798 @default.
- W2058746952 hasConceptScore W2058746952C55493867 @default.
- W2058746952 hasConceptScore W2058746952C58121356 @default.
- W2058746952 hasFunder F4320320912 @default.
- W2058746952 hasIssue "4" @default.
- W2058746952 hasLocation W20587469521 @default.
- W2058746952 hasLocation W20587469522 @default.
- W2058746952 hasOpenAccess W2058746952 @default.
- W2058746952 hasPrimaryLocation W20587469521 @default.
- W2058746952 hasRelatedWork W1968648371 @default.
- W2058746952 hasRelatedWork W1996718803 @default.
- W2058746952 hasRelatedWork W2025296270 @default.
- W2058746952 hasRelatedWork W2040991518 @default.
- W2058746952 hasRelatedWork W2058746952 @default.
- W2058746952 hasRelatedWork W2089162586 @default.
- W2058746952 hasRelatedWork W2265177709 @default.
- W2058746952 hasRelatedWork W229731679 @default.
- W2058746952 hasRelatedWork W328696666 @default.
- W2058746952 hasRelatedWork W4254744075 @default.
- W2058746952 hasVolume "90" @default.
- W2058746952 isParatext "false" @default.
- W2058746952 isRetracted "false" @default.
- W2058746952 magId "2058746952" @default.
- W2058746952 workType "article" @default.