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• W2058803605 abstract "Signal transduction pathways may have important regulatory roles in cellular events in the human parasite Schistosoma mansoni. The presence of the phosphoinositide response in S. mansoni was examined by radiolabeling intact worms with 20 muCi of [3H]myoinositol for 24 hr and stimulating parasites with 25 mM NaF and 10 microM AlCl3 in the presence of 10 mM LiCl. Total inositol phosphates were increased within 2 min and maximal accumulation was achieved after 30 min. Similar results were seen with the non-hydrolyzable GTP analogues GTP gamma S and GppNHp while only minimal changes were detected with GMP. Neomycin inhibited NaF-induced inositol phosphate production. NaF stimulated a significant 3.6-fold increase of inositol phosphates in females compared to males. These data suggest that stimulation of guanine nucleotide-binding regulatory proteins activates phospholipase C resulting in production of inositol phosphates in S. mansoni." @default.
• W2058803605 created "2016-06-24" @default.
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• W2058803605 date "1992-02-01" @default.
• W2058803605 modified "2023-09-24" @default.
• W2058803605 title "Schistosoma mansoni: Characterization of phosphoinositide response" @default.
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• W2058803605 doi "https://doi.org/10.1016/0014-4894(92)90137-y" @default.
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