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• W2058839641 abstract "Background This study was designed to establish a reference interval for sweat chloride for infants without evidence of cystic fibrosis (CF), aged between 5 wk and 6 wk, a time when sweat testing is an integral part of newborn screening for CF. In addition, we compared the gold standard method of sweat testing (quantitative pilocarpine iontophoresis [QPIT, coulometry]) with an emerging methodology (Macroduct™ [ISE]). Methods This was a prospective study on healthy infants at 5–6 wk of age. Sweat collection was undertaken at home on both outer thigh areas using two methods (QPIT and Macroduct™). The order of testing was randomly assigned. Filter paper samples (QPIT) were analysed using flame photometry and coulometry. Macroduct™ samples were analysed using ion-selective electrodes (ISE, Abbott Architect c8000, UK). Results Insufficient sweat was collected on 28 occasions with the QPIT (coulometry) method and on 31 with the Macroduct™ (ISE) capillary system. We achieved a 92% success rate in undertaking two sweat collections consecutively (n = 177). Sweat chloride concentrations were normally distributed with excellent limits of agreement between the two methods of sweat collection and analysis (n = 150). Median (IQR) sweat chloride was 11.2 mmol/L (8–13) with QPIT (coulometry) method with a 99.5th centile (n = 165) of 24 mmol/L. Conclusion The Macroduct™ (ISE) capillary sweat collection system is valid in this age group. Sweat chloride concentrations above 30 mmol/L should prompt assessment in a specialist CF centre." @default.
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• W2058839641 date "2009-01-01" @default.
• W2058839641 modified "2023-10-06" @default.
• W2058839641 title "A reference interval for sweat chloride in infants aged between five and six weeks of age" @default.
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• W2058839641 doi "https://doi.org/10.1258/acb.2008.008081" @default.
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