FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2058867182> ?p ?o ?g. }

• W2058867182 endingPage "e83833" @default.
• W2058867182 startingPage "e83833" @default.
• W2058867182 abstract "Background Stem/progenitor cells (SPCs) demonstrate neuro-regenerative potential that is dependent upon their humoral activity by producing various trophic factors regulating cell migration, growth, and differentiation. Herein, we compared the expression of neurotrophins (NTs) and their receptors in specific umbilical cord blood (UCB) SPC populations, including lineage-negative, CD34+, and CD133+ cells, with that in unsorted, nucleated cells (NCs). Methods and Results The expression of NTs and their receptors was detected by QRT-PCR, western blotting, and immunofluorescent staining in UCB-derived SPC populations (i.e., NCs vs. lineage-negative, CD34+, and CD133+ cells). To better characterize, global gene expression profiles of SPCs were determined using genome-wide RNA microarray technology. Furthermore, the intracellular production of crucial neuro-regenerative NTs (i.e., BDNF and NT-3) was assessed in NCs and lineage-negative cells after incubation for 24, 48, and 72 h in both serum and serum-free conditions. We discovered significantly higher expression of NTs and NT receptors at both the mRNA and protein level in lineage-negative, CD34+, and CD133+ cells than in NCs. Global gene expression analysis revealed considerably higher expression of genes associated with the production and secretion of proteins, migration, proliferation, and differentiation in lineage-negative cells than in CD34+ or CD133+ cell populations. Notably, after short-term incubation under serum-free conditions, lineage-negative cells and NCs produced significantly higher amounts of BDNF and NT-3 than under steady-state conditions. Finally, conditioned medium (CM) from lineage-negative SPCs exerted a beneficial impact on neural cell survival and proliferation. Conclusions Collectively, our findings demonstrate that UCB-derived SPCs highly express NTs and their relevant receptors under steady-state conditions, NT expression is greater under stress-related conditions and that CM from SPCs favorable influence neural cell proliferation and survival. Understanding the mechanisms governing the characterization and humoral activity of subsets of SPCs may yield new therapeutic strategies that might be more effective in treating neurodegenerative disorders." @default.
• W2058867182 created "2016-06-24" @default.
• W2058867182 creator A5004839255 @default.
• W2058867182 creator A5014971780 @default.
• W2058867182 creator A5015206786 @default.
• W2058867182 creator A5026979519 @default.
• W2058867182 creator A5044585605 @default.
• W2058867182 creator A5047321589 @default.
• W2058867182 creator A5073881948 @default.
• W2058867182 creator A5075634968 @default.
• W2058867182 creator A5090406221 @default.
• W2058867182 date "2013-12-31" @default.
• W2058867182 modified "2023-09-29" @default.
• W2058867182 title "Humoral Activity of Cord Blood-Derived Stem/Progenitor Cells: Implications for Stem Cell-Based Adjuvant Therapy of Neurodegenerative Disorders" @default.
• W2058867182 cites W116732461 @default.
• W2058867182 cites W1898701513 @default.
• W2058867182 cites W1966545752 @default.
• W2058867182 cites W1970093663 @default.
• W2058867182 cites W1977782116 @default.
• W2058867182 cites W1980357664 @default.
• W2058867182 cites W1985886057 @default.
• W2058867182 cites W1987555163 @default.
• W2058867182 cites W1994935161 @default.
• W2058867182 cites W2003649512 @default.
• W2058867182 cites W2006376003 @default.
• W2058867182 cites W2011325117 @default.
• W2058867182 cites W2013709298 @default.
• W2058867182 cites W2016208861 @default.
• W2058867182 cites W2021682420 @default.
• W2058867182 cites W2022194525 @default.
• W2058867182 cites W2026724187 @default.
• W2058867182 cites W2028535131 @default.
• W2058867182 cites W2031691983 @default.
• W2058867182 cites W2033045687 @default.
• W2058867182 cites W2043415961 @default.
• W2058867182 cites W2050381480 @default.
• W2058867182 cites W2053177057 @default.
• W2058867182 cites W2053704719 @default.
• W2058867182 cites W2054880213 @default.
• W2058867182 cites W2059014353 @default.
• W2058867182 cites W2062221785 @default.
• W2058867182 cites W2065716392 @default.
• W2058867182 cites W2068552089 @default.
• W2058867182 cites W2073523882 @default.
• W2058867182 cites W2073678538 @default.
• W2058867182 cites W2080666748 @default.
• W2058867182 cites W2083764625 @default.
• W2058867182 cites W2086336982 @default.
• W2058867182 cites W2087672059 @default.
• W2058867182 cites W2103845617 @default.
• W2058867182 cites W2110819175 @default.
• W2058867182 cites W2119144139 @default.
• W2058867182 cites W2120819751 @default.
• W2058867182 cites W2124736946 @default.
• W2058867182 cites W2127447774 @default.
• W2058867182 cites W2131963545 @default.
• W2058867182 cites W2138909102 @default.
• W2058867182 cites W2139565721 @default.
• W2058867182 cites W2157632958 @default.
• W2058867182 cites W2158733041 @default.
• W2058867182 cites W2160327761 @default.
• W2058867182 cites W2160829574 @default.
• W2058867182 cites W2161661825 @default.
• W2058867182 cites W2165695867 @default.
• W2058867182 cites W2166508103 @default.
• W2058867182 cites W2169290971 @default.
• W2058867182 cites W2334937945 @default.
• W2058867182 cites W2416198997 @default.
• W2058867182 cites W4254329016 @default.
• W2058867182 cites W4254868516 @default.
• W2058867182 doi "https://doi.org/10.1371/journal.pone.0083833" @default.
• W2058867182 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3877125" @default.
• W2058867182 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24391835" @default.
• W2058867182 hasPublicationYear "2013" @default.
• W2058867182 type Work @default.
• W2058867182 sameAs 2058867182 @default.
• W2058867182 citedByCount "34" @default.
• W2058867182 countsByYear W20588671822014 @default.
• W2058867182 countsByYear W20588671822015 @default.
• W2058867182 countsByYear W20588671822016 @default.
• W2058867182 countsByYear W20588671822017 @default.
• W2058867182 countsByYear W20588671822018 @default.
• W2058867182 countsByYear W20588671822019 @default.
• W2058867182 countsByYear W20588671822020 @default.
• W2058867182 countsByYear W20588671822021 @default.
• W2058867182 countsByYear W20588671822022 @default.
• W2058867182 countsByYear W20588671822023 @default.
• W2058867182 crossrefType "journal-article" @default.
• W2058867182 hasAuthorship W2058867182A5004839255 @default.
• W2058867182 hasAuthorship W2058867182A5014971780 @default.
• W2058867182 hasAuthorship W2058867182A5015206786 @default.
• W2058867182 hasAuthorship W2058867182A5026979519 @default.
• W2058867182 hasAuthorship W2058867182A5044585605 @default.
• W2058867182 hasAuthorship W2058867182A5047321589 @default.
• W2058867182 hasAuthorship W2058867182A5073881948 @default.
• W2058867182 hasAuthorship W2058867182A5075634968 @default.
• W2058867182 hasAuthorship W2058867182A5090406221 @default.
• W2058867182 hasBestOaLocation W20588671821 @default.

• next