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• W2058883908 abstract "ABSTRACT The surface physicochemical properties of Listeria monocytogenes LO28 under different conditions (temperature and growth phase) were determined by use of microelectrophoresis and microbial adhesion to solvents. The effect of these parameters on adhesion and biofilm formation by L. monocytogenes LO28 on hydrophilic (stainless steel) and hydrophobic (polytetrafluoroethylene [PTFE]) surfaces was assessed. The bacterial cells were always negatively charged and possessed hydrophilic surface properties, which were negatively correlated with growth temperature. The colonization of the two surfaces, monitored by scanning electron microscopy, epifluorescence microscopy, and cell enumeration, showed that the strain had a great capacity to colonize both surfaces whatever the incubation temperature. However, biofilm formation was faster on the hydrophilic substratum. After 5 days at 37 or 20°C, the biofilm structure was composed of aggregates with a three-dimensional shape, but significant detachment took place on PTFE at 37°C. At 8°C, only a bacterial monolayer was visible on stainless steel, while no growth was observed on PTFE. The growth phase of bacteria used to inoculate surfaces had a significant effect only in some cases during the first steps of biofilm formation. The surface physicochemical properties of the strain are correlated with adhesion and surface colonization." @default.
• W2058883908 created "2016-06-24" @default.
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• W2058883908 date "2002-02-01" @default.
• W2058883908 modified "2023-10-06" @default.
• W2058883908 title "<i>Listeria monocytogenes</i> LO28: Surface Physicochemical Properties and Ability To Form Biofilms at Different Temperatures and Growth Phases" @default.
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• W2058883908 doi "https://doi.org/10.1128/aem.68.2.728-737.2002" @default.
• W2058883908 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/126664" @default.
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