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• W2058902441 abstract "Artemisinin isolated from the aerial parts of Artemisia annua L., is a promising and potent antimalarial drug, which meets the dual challenge posed by drug-resistant parasites and rapid progression of malarial illness. The aim of the current study was to develop a reliable and fast analytical procedure for the determination of artemisinin in A. annua using high performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD) in couple with microwave-assisted extraction (MAE) as an efficient sample preparation technique. The HPLC conditions were Agilent C18 column using water:acetonitrile (40:60 v/v) mixture as mobile phase at a flow rate of 1 mL min−1. ELSD conditions were optimized at nebulizer-gas flow rate of 2.0 L min−1 and drift tube temperature of 70 °C under the impactor off-mode, and the gain was set at 2. Afterwards, method validation system for HPLC–ELSD analysis was developed. Calibration range was 0.2–1.0 mg mL−1 and correlation coefficient r was above 0.9990. Precision experiments showed relative standard deviation (R.S.D.) of retention time was less than 0.5% and R.S.D. of peak area was less than 1.30%. Inter-day and intra-day variabilities showed that R.S.D. was ranged from 1.01% to 4.66%. Limit of detection was less than 40 μg mL−1 and limit of quantification was less than 100 μg mL−1. Accuracy validation showed that average recovery was between 98.23% and 104.97%. The developed analytical procedure was successfully applied to determine the contents of artemisinin in the different parts of A. annua plants." @default.
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• W2058902441 date "2007-01-01" @default.
• W2058902441 modified "2023-09-26" @default.
• W2058902441 title "An effective method for fast determination of artemisinin in Artemisia annua L. by high performance liquid chromatography with evaporative light scattering detection" @default.
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• W2058902441 doi "https://doi.org/10.1016/j.aca.2006.08.038" @default.
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