Matches in SemOpenAlex for { <https://semopenalex.org/work/W2058906522> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2058906522 endingPage "143" @default.
- W2058906522 startingPage "134" @default.
- W2058906522 abstract "Eicosanoids and platelet-activating factor (PAF) are phospholipid-derived lipid mediators produced by various tissues and cells through a cascade pathway. For a comprehensive analysis of these lipid mediators, a simultaneous quantitation method with sensitivity and reliability is necessary. This article details a development of column-switching reversed-phase liquid chromatography-tandem mass spectrometry for multiplex quantitation of eicosanoids and PAF. The adsorptive nature of lipids caused significant loss of signal in a conventional column-switching configuration. The use of an online-dilution method allowed use of 100% methanol as a sample solvent, which prevented sample adsorption to contacting surfaces. Addition of 0.2% formic acid to the sample solvent was required for the successful introduction of LTC4 to the trapping column and minimizing its carryover. The optimized method provided rapid analysis of 14 lipid mediators with a throughput of 96 samples/24 h, lower limits of quantitation of 5 pg on column, and linear calibration ranges up to 2000-5000 pg. The system was highly compatible with solid-phase-extracted samples, as methanol-eluted fractions were directly injected without reconstitution. The analysis of lipid mediator production of macrophage-like RAW264.7 cells demonstrated that the cell-based assay can be performed in a 96-well format, suitable for metabolomics analyses and/or screening strategies." @default.
- W2058906522 created "2016-06-24" @default.
- W2058906522 creator A5000716531 @default.
- W2058906522 creator A5008934626 @default.
- W2058906522 creator A5036938042 @default.
- W2058906522 creator A5067463702 @default.
- W2058906522 date "2005-07-01" @default.
- W2058906522 modified "2023-10-08" @default.
- W2058906522 title "A multiplex quantitation method for eicosanoids and platelet-activating factor using column-switching reversed-phase liquid chromatography–tandem mass spectrometry" @default.
- W2058906522 cites W1971499375 @default.
- W2058906522 cites W1988842726 @default.
- W2058906522 cites W2000983103 @default.
- W2058906522 cites W2002845962 @default.
- W2058906522 cites W2004611367 @default.
- W2058906522 cites W2007187686 @default.
- W2058906522 cites W2055565478 @default.
- W2058906522 cites W2063956233 @default.
- W2058906522 cites W2065971374 @default.
- W2058906522 cites W2073202677 @default.
- W2058906522 cites W2083856546 @default.
- W2058906522 cites W2090883822 @default.
- W2058906522 cites W2093223464 @default.
- W2058906522 cites W2132305492 @default.
- W2058906522 cites W2146892797 @default.
- W2058906522 cites W2158960911 @default.
- W2058906522 cites W4240627486 @default.
- W2058906522 doi "https://doi.org/10.1016/j.ab.2005.03.048" @default.
- W2058906522 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15958190" @default.
- W2058906522 hasPublicationYear "2005" @default.
- W2058906522 type Work @default.
- W2058906522 sameAs 2058906522 @default.
- W2058906522 citedByCount "113" @default.
- W2058906522 countsByYear W20589065222012 @default.
- W2058906522 countsByYear W20589065222013 @default.
- W2058906522 countsByYear W20589065222014 @default.
- W2058906522 countsByYear W20589065222015 @default.
- W2058906522 countsByYear W20589065222016 @default.
- W2058906522 countsByYear W20589065222017 @default.
- W2058906522 countsByYear W20589065222018 @default.
- W2058906522 countsByYear W20589065222019 @default.
- W2058906522 countsByYear W20589065222020 @default.
- W2058906522 countsByYear W20589065222021 @default.
- W2058906522 countsByYear W20589065222022 @default.
- W2058906522 countsByYear W20589065222023 @default.
- W2058906522 crossrefType "journal-article" @default.
- W2058906522 hasAuthorship W2058906522A5000716531 @default.
- W2058906522 hasAuthorship W2058906522A5008934626 @default.
- W2058906522 hasAuthorship W2058906522A5036938042 @default.
- W2058906522 hasAuthorship W2058906522A5067463702 @default.
- W2058906522 hasConcept C162356407 @default.
- W2058906522 hasConcept C179998833 @default.
- W2058906522 hasConcept C185592680 @default.
- W2058906522 hasConcept C2778576202 @default.
- W2058906522 hasConcept C31827203 @default.
- W2058906522 hasConcept C43617362 @default.
- W2058906522 hasConcept C45188465 @default.
- W2058906522 hasConcept C64489805 @default.
- W2058906522 hasConcept C73826308 @default.
- W2058906522 hasConceptScore W2058906522C162356407 @default.
- W2058906522 hasConceptScore W2058906522C179998833 @default.
- W2058906522 hasConceptScore W2058906522C185592680 @default.
- W2058906522 hasConceptScore W2058906522C2778576202 @default.
- W2058906522 hasConceptScore W2058906522C31827203 @default.
- W2058906522 hasConceptScore W2058906522C43617362 @default.
- W2058906522 hasConceptScore W2058906522C45188465 @default.
- W2058906522 hasConceptScore W2058906522C64489805 @default.
- W2058906522 hasConceptScore W2058906522C73826308 @default.
- W2058906522 hasFunder F4320320912 @default.
- W2058906522 hasIssue "1" @default.
- W2058906522 hasLocation W20589065221 @default.
- W2058906522 hasLocation W20589065222 @default.
- W2058906522 hasOpenAccess W2058906522 @default.
- W2058906522 hasPrimaryLocation W20589065221 @default.
- W2058906522 hasRelatedWork W1968381290 @default.
- W2058906522 hasRelatedWork W1968441093 @default.
- W2058906522 hasRelatedWork W1985701257 @default.
- W2058906522 hasRelatedWork W2027143713 @default.
- W2058906522 hasRelatedWork W2035251241 @default.
- W2058906522 hasRelatedWork W2311474148 @default.
- W2058906522 hasRelatedWork W2318291238 @default.
- W2058906522 hasRelatedWork W4200407455 @default.
- W2058906522 hasRelatedWork W4323347615 @default.
- W2058906522 hasRelatedWork W2094644704 @default.
- W2058906522 hasVolume "342" @default.
- W2058906522 isParatext "false" @default.
- W2058906522 isRetracted "false" @default.
- W2058906522 magId "2058906522" @default.
- W2058906522 workType "article" @default.