FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2058906671> ?p ?o ?g. }

• W2058906671 endingPage "249" @default.
• W2058906671 startingPage "243" @default.
• W2058906671 abstract "In this study we have examined the cholesterol oxidase (Streptomyces cinnamomeus) catalyzed conversion of either 5-cholesten-3β-ol or 5-cholesten-3-one into 4-cholesten-3-one in pure sterol or mixed phospholipid-containing monolayers at the air/buffer interface. The mean molecular area requirement of 5-cholesten-3-one in a pure monolayer was slightly smaller than the comparable area required by 5-cholesten-3β-ol (although the collapse pressure was markedly lower for 5-cholesten-3-one), and both sterols were about equally capable of condensing the lateral packing density of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine at a lateral surface pressure of 20 mN/m. Both sterols were converted by cholesterol oxidase to 4-cholesten-3-one, the reaction being faster with 5-cholesten-3-one as compared to 5-cholesten-3β-ol. When the temperature-dependency of the cholesterol oxidase catalyzed conversion of the sterols to 4-cholesten-3-one was examined, the Arrhenius activation energy was calculated to+ 30 kJ/mol and + 27 kJ/mol for 5-cholesten-3β-ol and 5-cholesten-3-one, respectively, when the sterols were presented to the enzyme as pure sterol monolayers at a lateral surface pressure of 20 mN/m. With a mixed monolayer containing 40 mol% sterol and 60 mol% EPC, the corresponding activation energies were + 107 kJ/mol and + 96 kJ/mol for 5-cholesten-3β-ol and 5-cholesten-3-one, respectively. With the monolayer system used, it appeared that the over all rate-limiting step in the enzyme-catalyzed conversion of 5-en-sterols to 4-en-3-one was the desorption of the sterol molecules from the monolayer into the active site of the enzyme at the interface. This appeared to be true both with pure sterol monolayers as well as with mixed monolayers containing phosphatidylcholine." @default.
• W2058906671 created "2016-06-24" @default.
• W2058906671 creator A5002705599 @default.
• W2058906671 creator A5048160824 @default.
• W2058906671 date "1993-02-01" @default.
• W2058906671 modified "2023-09-29" @default.
• W2058906671 title "Oxidation/isomerization of 5-cholesten-3β-ol and 5-cholesten-3-one to 4-cholesten-3-one in pure sterol and mixed phospholipid-containing monolayers by cholesterol oxidase" @default.
• W2058906671 cites W1491842649 @default.
• W2058906671 cites W1546989961 @default.
• W2058906671 cites W1899257984 @default.
• W2058906671 cites W1951207131 @default.
• W2058906671 cites W1975821967 @default.
• W2058906671 cites W1980400254 @default.
• W2058906671 cites W1980750564 @default.
• W2058906671 cites W1996380871 @default.
• W2058906671 cites W1996385047 @default.
• W2058906671 cites W1997901653 @default.
• W2058906671 cites W2017484470 @default.
• W2058906671 cites W2019789194 @default.
• W2058906671 cites W2025945108 @default.
• W2058906671 cites W2030724380 @default.
• W2058906671 cites W2031802370 @default.
• W2058906671 cites W2039694381 @default.
• W2058906671 cites W2043576743 @default.
• W2058906671 cites W2043952833 @default.
• W2058906671 cites W2049266592 @default.
• W2058906671 cites W2058471523 @default.
• W2058906671 cites W2058507039 @default.
• W2058906671 cites W2089062898 @default.
• W2058906671 cites W2131496307 @default.
• W2058906671 cites W2154995837 @default.
• W2058906671 cites W2163666564 @default.
• W2058906671 cites W2248522108 @default.
• W2058906671 cites W2256067245 @default.
• W2058906671 cites W2314009237 @default.
• W2058906671 cites W2333032100 @default.
• W2058906671 cites W95023376 @default.
• W2058906671 doi "https://doi.org/10.1016/0005-2736(93)90295-b" @default.
• W2058906671 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8431456" @default.
• W2058906671 hasPublicationYear "1993" @default.
• W2058906671 type Work @default.
• W2058906671 sameAs 2058906671 @default.
• W2058906671 citedByCount "13" @default.
• W2058906671 countsByYear W20589066712014 @default.
• W2058906671 countsByYear W20589066712016 @default.
• W2058906671 countsByYear W20589066712023 @default.
• W2058906671 crossrefType "journal-article" @default.
• W2058906671 hasAuthorship W2058906671A5002705599 @default.
• W2058906671 hasAuthorship W2058906671A5048160824 @default.
• W2058906671 hasConcept C185592680 @default.
• W2058906671 hasConcept C2778163477 @default.
• W2058906671 hasConcept C2778718757 @default.
• W2058906671 hasConcept C2779387492 @default.
• W2058906671 hasConcept C55493867 @default.
• W2058906671 hasConcept C7070889 @default.
• W2058906671 hasConcept C86803240 @default.
• W2058906671 hasConceptScore W2058906671C185592680 @default.
• W2058906671 hasConceptScore W2058906671C2778163477 @default.
• W2058906671 hasConceptScore W2058906671C2778718757 @default.
• W2058906671 hasConceptScore W2058906671C2779387492 @default.
• W2058906671 hasConceptScore W2058906671C55493867 @default.
• W2058906671 hasConceptScore W2058906671C7070889 @default.
• W2058906671 hasConceptScore W2058906671C86803240 @default.
• W2058906671 hasIssue "2" @default.
• W2058906671 hasLocation W20589066711 @default.
• W2058906671 hasLocation W20589066712 @default.
• W2058906671 hasOpenAccess W2058906671 @default.
• W2058906671 hasPrimaryLocation W20589066711 @default.
• W2058906671 hasRelatedWork W1964749637 @default.
• W2058906671 hasRelatedWork W2007268251 @default.
• W2058906671 hasRelatedWork W2043952833 @default.
• W2058906671 hasRelatedWork W2049705078 @default.
• W2058906671 hasRelatedWork W2077788491 @default.
• W2058906671 hasRelatedWork W2092735428 @default.
• W2058906671 hasRelatedWork W2163666564 @default.
• W2058906671 hasRelatedWork W2282375391 @default.
• W2058906671 hasRelatedWork W2414354016 @default.
• W2058906671 hasRelatedWork W4253935212 @default.
• W2058906671 hasVolume "1145" @default.
• W2058906671 isParatext "false" @default.
• W2058906671 isRetracted "false" @default.
• W2058906671 magId "2058906671" @default.
• W2058906671 workType "article" @default.