FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2058907547> ?p ?o ?g. }

• W2058907547 endingPage "26932" @default.
• W2058907547 startingPage "26923" @default.
• W2058907547 abstract "p190RhoGAP-A (p190) is a GTPase-activating protein known to regulate actin cytoskeleton dynamics by decreasing RhoGTP levels through activation of Rho intrinsic GTPase activity. We have previously shown that p190 protein levels are cell cycle-regulated, decreasing in mitosis, and that this decrease is mediated by the ubiquitin-proteasome pathway. In addition, overexpression of p190 results in decreased RhoGTP levels at the cleavage furrow during cytokinesis, p190 and the RhoGEF Ect2 play opposing roles in cytokinesis, and sustained levels of p190 in mitosis are associated with cytokinesis failure, all findings that suggest but do not directly demonstrate that completion of cytokinesis is dependent on reduced levels of p190. Here we report, using an RNAi reconstitution approach with a degradation-resistant mutant, that decreased p190 levels are required for successful cytokinesis. We also show that the multinucleation phenotype is dependent on p190 RhoGAP activity, determine that the N-terminal GBDS1 region is necessary and sufficient for p190 mitotic ubiquitination and degradation, and identify four N-terminal residues as necessary for the degradation of p190 in mitosis. Our data indicate that in addition to activation of RhoGEF(s), reduction of RhoGAP (p190) is a critical mechanism by which increased RhoGTP levels are achieved in late mitosis, thereby ensuring proper cell division. p190RhoGAP-A (p190) is a GTPase-activating protein known to regulate actin cytoskeleton dynamics by decreasing RhoGTP levels through activation of Rho intrinsic GTPase activity. We have previously shown that p190 protein levels are cell cycle-regulated, decreasing in mitosis, and that this decrease is mediated by the ubiquitin-proteasome pathway. In addition, overexpression of p190 results in decreased RhoGTP levels at the cleavage furrow during cytokinesis, p190 and the RhoGEF Ect2 play opposing roles in cytokinesis, and sustained levels of p190 in mitosis are associated with cytokinesis failure, all findings that suggest but do not directly demonstrate that completion of cytokinesis is dependent on reduced levels of p190. Here we report, using an RNAi reconstitution approach with a degradation-resistant mutant, that decreased p190 levels are required for successful cytokinesis. We also show that the multinucleation phenotype is dependent on p190 RhoGAP activity, determine that the N-terminal GBDS1 region is necessary and sufficient for p190 mitotic ubiquitination and degradation, and identify four N-terminal residues as necessary for the degradation of p190 in mitosis. Our data indicate that in addition to activation of RhoGEF(s), reduction of RhoGAP (p190) is a critical mechanism by which increased RhoGTP levels are achieved in late mitosis, thereby ensuring proper cell division." @default.
• W2058907547 created "2016-06-24" @default.
• W2058907547 creator A5002041109 @default.
• W2058907547 creator A5010477060 @default.
• W2058907547 creator A5011870485 @default.
• W2058907547 creator A5026405192 @default.
• W2058907547 creator A5059530747 @default.
• W2058907547 creator A5070518506 @default.
• W2058907547 creator A5088634138 @default.
• W2058907547 date "2010-08-01" @default.
• W2058907547 modified "2023-09-29" @default.
• W2058907547 title "Mitotic Down-regulation of p190RhoGAP Is Required for the Successful Completion of Cytokinesis" @default.
• W2058907547 cites W108705584 @default.
• W2058907547 cites W1913395666 @default.
• W2058907547 cites W1966855723 @default.
• W2058907547 cites W1971584907 @default.
• W2058907547 cites W1971589188 @default.
• W2058907547 cites W1971831085 @default.
• W2058907547 cites W1978032395 @default.
• W2058907547 cites W1995405908 @default.
• W2058907547 cites W1998143618 @default.
• W2058907547 cites W1998645764 @default.
• W2058907547 cites W2011833766 @default.
• W2058907547 cites W2018307646 @default.
• W2058907547 cites W2030341081 @default.
• W2058907547 cites W2031172767 @default.
• W2058907547 cites W2036367334 @default.
• W2058907547 cites W2042221256 @default.
• W2058907547 cites W2050892933 @default.
• W2058907547 cites W2063328565 @default.
• W2058907547 cites W2063767869 @default.
• W2058907547 cites W2067024225 @default.
• W2058907547 cites W2070326039 @default.
• W2058907547 cites W2080151197 @default.
• W2058907547 cites W2080374319 @default.
• W2058907547 cites W2083108636 @default.
• W2058907547 cites W2083514445 @default.
• W2058907547 cites W2084533564 @default.
• W2058907547 cites W2086061852 @default.
• W2058907547 cites W2089950231 @default.
• W2058907547 cites W2090821655 @default.
• W2058907547 cites W2091849418 @default.
• W2058907547 cites W2105499419 @default.
• W2058907547 cites W2105630217 @default.
• W2058907547 cites W2108641962 @default.
• W2058907547 cites W2116463110 @default.
• W2058907547 cites W2123649435 @default.
• W2058907547 cites W2134807132 @default.
• W2058907547 cites W2140858258 @default.
• W2058907547 cites W2148997468 @default.
• W2058907547 cites W2150125913 @default.
• W2058907547 cites W2151065520 @default.
• W2058907547 cites W2161614836 @default.
• W2058907547 cites W2167324829 @default.
• W2058907547 cites W2170963117 @default.
• W2058907547 cites W2408556838 @default.
• W2058907547 doi "https://doi.org/10.1074/jbc.m110.103804" @default.
• W2058907547 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2930692" @default.
• W2058907547 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20534586" @default.
• W2058907547 hasPublicationYear "2010" @default.
• W2058907547 type Work @default.
• W2058907547 sameAs 2058907547 @default.
• W2058907547 citedByCount "23" @default.
• W2058907547 countsByYear W20589075472012 @default.
• W2058907547 countsByYear W20589075472013 @default.
• W2058907547 countsByYear W20589075472014 @default.
• W2058907547 countsByYear W20589075472016 @default.
• W2058907547 countsByYear W20589075472017 @default.
• W2058907547 countsByYear W20589075472018 @default.
• W2058907547 countsByYear W20589075472020 @default.
• W2058907547 crossrefType "journal-article" @default.
• W2058907547 hasAuthorship W2058907547A5002041109 @default.
• W2058907547 hasAuthorship W2058907547A5010477060 @default.
• W2058907547 hasAuthorship W2058907547A5011870485 @default.
• W2058907547 hasAuthorship W2058907547A5026405192 @default.
• W2058907547 hasAuthorship W2058907547A5059530747 @default.
• W2058907547 hasAuthorship W2058907547A5070518506 @default.
• W2058907547 hasAuthorship W2058907547A5088634138 @default.
• W2058907547 hasBestOaLocation W20589075471 @default.
• W2058907547 hasConcept C1491633281 @default.
• W2058907547 hasConcept C207332259 @default.
• W2058907547 hasConcept C29537977 @default.
• W2058907547 hasConcept C45472230 @default.
• W2058907547 hasConcept C53420372 @default.
• W2058907547 hasConcept C54355233 @default.
• W2058907547 hasConcept C6391034 @default.
• W2058907547 hasConcept C85813293 @default.
• W2058907547 hasConcept C86803240 @default.
• W2058907547 hasConcept C93304396 @default.
• W2058907547 hasConcept C95444343 @default.
• W2058907547 hasConceptScore W2058907547C1491633281 @default.
• W2058907547 hasConceptScore W2058907547C207332259 @default.
• W2058907547 hasConceptScore W2058907547C29537977 @default.
• W2058907547 hasConceptScore W2058907547C45472230 @default.
• W2058907547 hasConceptScore W2058907547C53420372 @default.
• W2058907547 hasConceptScore W2058907547C54355233 @default.
• W2058907547 hasConceptScore W2058907547C6391034 @default.
• W2058907547 hasConceptScore W2058907547C85813293 @default.

• next