FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2058960751> ?p ?o ?g. }

• W2058960751 abstract "Characteristics and activities of estrone sulfate (E1S) and dehydroepiandrosterone sulfate (DHAS) sulfatases were studied in epithelium and stroma of benign hyperplastic tissues from human prostates. Tissues were obtained by suprapubic prostatectomy, and epithelium and stroma were separated mechanically by standard techniques. The assay procedure comprised homogenization in Tris-buffer, incubation of the homogenate with [3H]E1S or [3H]DHAS, separation of free steroids from nonhydrolyzed steroid sulfates by extraction with ether, and their final quantification by LSC. The main results were: (1) The pH-optimum of the sulfatase was found at pH 7.0. (2) The highest specific sulfatase activity was found in the epithelium and was associated with its nuclear fraction. (3) Michaelis-Menten constants Km (microM) were 8.7 +/- 1.4 (7) and 4.3 +/- 0.8 (5), maximum velocity rates Vmax (nmol/h x mgDNA) were 47.4 +/- 8.8 (7) and 8.4 +/- 1.5 (5) for E1S and DHAS, respectively (means +/- SEM (n]. (4) The enzymatic cleavage of E1-sulfate was competitively inhibited by DHA-sulfate and vice versa with inhibition constants Ki (microM) of 4.0 +/- 0.5 (2) for E1S and 2.7 +/- 0.4 (2) for DHAS. On the basis of these findings, possible roles of steroid sulfate-sulfatases in forming precursors of active androgens and estrogens from the high amounts of E1S and DHAS in blood are discussed." @default.
• W2058960751 created "2016-06-24" @default.
• W2058960751 creator A5017654933 @default.
• W2058960751 creator A5038480337 @default.
• W2058960751 creator A5056611032 @default.
• W2058960751 date "1989-08-01" @default.
• W2058960751 modified "2023-09-26" @default.
• W2058960751 title "Steroid sulfate sulfatase in human benign prostatic hyperplasia: Characterization and quantification of the enzyme in epithelium and stroma" @default.
• W2058960751 cites W1885778179 @default.
• W2058960751 cites W1966755583 @default.
• W2058960751 cites W1977189077 @default.
• W2058960751 cites W1977333419 @default.
• W2058960751 cites W1980545656 @default.
• W2058960751 cites W1982098061 @default.
• W2058960751 cites W1987891693 @default.
• W2058960751 cites W1989397789 @default.
• W2058960751 cites W1992345828 @default.
• W2058960751 cites W2003447742 @default.
• W2058960751 cites W2008625314 @default.
• W2058960751 cites W2010243200 @default.
• W2058960751 cites W2020218903 @default.
• W2058960751 cites W2022896834 @default.
• W2058960751 cites W2024940566 @default.
• W2058960751 cites W2032606782 @default.
• W2058960751 cites W2054979333 @default.
• W2058960751 cites W2060518858 @default.
• W2058960751 cites W2062089709 @default.
• W2058960751 cites W2063004592 @default.
• W2058960751 cites W2066942845 @default.
• W2058960751 cites W2074333008 @default.
• W2058960751 cites W2074703961 @default.
• W2058960751 cites W2076747943 @default.
• W2058960751 cites W2077118870 @default.
• W2058960751 cites W2079328604 @default.
• W2058960751 cites W2086780680 @default.
• W2058960751 cites W2091860519 @default.
• W2058960751 cites W2092195423 @default.
• W2058960751 cites W2101325314 @default.
• W2058960751 cites W2110401213 @default.
• W2058960751 doi "https://doi.org/10.1016/0022-4731(89)90294-x" @default.
• W2058960751 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2475673" @default.
• W2058960751 hasPublicationYear "1989" @default.
• W2058960751 type Work @default.
• W2058960751 sameAs 2058960751 @default.
• W2058960751 citedByCount "33" @default.
• W2058960751 countsByYear W20589607512012 @default.
• W2058960751 countsByYear W20589607512013 @default.
• W2058960751 countsByYear W20589607512015 @default.
• W2058960751 countsByYear W20589607512016 @default.
• W2058960751 countsByYear W20589607512018 @default.
• W2058960751 countsByYear W20589607512019 @default.
• W2058960751 countsByYear W20589607512020 @default.
• W2058960751 countsByYear W20589607512021 @default.
• W2058960751 crossrefType "journal-article" @default.
• W2058960751 hasAuthorship W2058960751A5017654933 @default.
• W2058960751 hasAuthorship W2058960751A5038480337 @default.
• W2058960751 hasAuthorship W2058960751A5056611032 @default.
• W2058960751 hasConcept C126322002 @default.
• W2058960751 hasConcept C134018914 @default.
• W2058960751 hasConcept C142724271 @default.
• W2058960751 hasConcept C155138218 @default.
• W2058960751 hasConcept C178790620 @default.
• W2058960751 hasConcept C181199279 @default.
• W2058960751 hasConcept C185592680 @default.
• W2058960751 hasConcept C204232928 @default.
• W2058960751 hasConcept C2777911890 @default.
• W2058960751 hasConcept C2778090324 @default.
• W2058960751 hasConcept C2778343803 @default.
• W2058960751 hasConcept C2780657110 @default.
• W2058960751 hasConcept C2780871851 @default.
• W2058960751 hasConcept C2780902042 @default.
• W2058960751 hasConcept C43617362 @default.
• W2058960751 hasConcept C52124034 @default.
• W2058960751 hasConcept C529295009 @default.
• W2058960751 hasConcept C55493867 @default.
• W2058960751 hasConcept C71315377 @default.
• W2058960751 hasConcept C71924100 @default.
• W2058960751 hasConcept C86803240 @default.
• W2058960751 hasConceptScore W2058960751C126322002 @default.
• W2058960751 hasConceptScore W2058960751C134018914 @default.
• W2058960751 hasConceptScore W2058960751C142724271 @default.
• W2058960751 hasConceptScore W2058960751C155138218 @default.
• W2058960751 hasConceptScore W2058960751C178790620 @default.
• W2058960751 hasConceptScore W2058960751C181199279 @default.
• W2058960751 hasConceptScore W2058960751C185592680 @default.
• W2058960751 hasConceptScore W2058960751C204232928 @default.
• W2058960751 hasConceptScore W2058960751C2777911890 @default.
• W2058960751 hasConceptScore W2058960751C2778090324 @default.
• W2058960751 hasConceptScore W2058960751C2778343803 @default.
• W2058960751 hasConceptScore W2058960751C2780657110 @default.
• W2058960751 hasConceptScore W2058960751C2780871851 @default.
• W2058960751 hasConceptScore W2058960751C2780902042 @default.
• W2058960751 hasConceptScore W2058960751C43617362 @default.
• W2058960751 hasConceptScore W2058960751C52124034 @default.
• W2058960751 hasConceptScore W2058960751C529295009 @default.
• W2058960751 hasConceptScore W2058960751C55493867 @default.
• W2058960751 hasConceptScore W2058960751C71315377 @default.
• W2058960751 hasConceptScore W2058960751C71924100 @default.
• W2058960751 hasConceptScore W2058960751C86803240 @default.
• W2058960751 hasLocation W20589607511 @default.

• next