Matches in SemOpenAlex for { <https://semopenalex.org/work/W2058967072> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2058967072 endingPage "6" @default.
- W2058967072 startingPage "1" @default.
- W2058967072 abstract "The major capsid protein L1 of papillomaviruses expressed recombinantly or in infected cells has the intrinsic ability to form virus-like particles (VLPs) which display conformational epitopes necessary to elicit high-titered, virus-neutralizing antibodies. We have shown previously that the L1 gene of human papillomavirus type 6a (HPV6) can be expressed efficiently in Saccharomyces cerevisae (Sc) as VLPs. However, when we attempted to express the L1 gene cloned from the closely related HPV11 in yeast, few VLPs were observed in crude lysates. The lower expression level of HPV11 L1 protein was found to result from a truncation of the HPV11 L1 mRNA. Since sequence requirements for transcriptional termination in yeast are still unclear, the HPV6 L1 gene was used as the basis for the complete synthetic reconstruction of the entire 1506-bp HPV11 L1 gene. Expression of this HPV6/11 hybrid L1 gene in yeast resulted in predominantly full-length L1 mRNA and a > 7-fold increased level of production of HPV11 VLPs compared to that expressed by the wild-type HPV11 L1 gene. The VLPs were shown to display the conformational epitopes important to elicit virus-neutralizing antibodies" @default.
- W2058967072 created "2016-06-24" @default.
- W2058967072 creator A5044292989 @default.
- W2058967072 creator A5070917422 @default.
- W2058967072 creator A5076921040 @default.
- W2058967072 date "1996-01-01" @default.
- W2058967072 modified "2023-10-14" @default.
- W2058967072 title "Expression of the major capsid protein of human papillomavirus type 11 in Saccharomyces cerevisae" @default.
- W2058967072 cites W1481736392 @default.
- W2058967072 cites W1500130815 @default.
- W2058967072 cites W1519578291 @default.
- W2058967072 cites W1616497959 @default.
- W2058967072 cites W1807715620 @default.
- W2058967072 cites W1947118328 @default.
- W2058967072 cites W1949036755 @default.
- W2058967072 cites W1985584979 @default.
- W2058967072 cites W1996010519 @default.
- W2058967072 cites W1997826177 @default.
- W2058967072 cites W2003676968 @default.
- W2058967072 cites W2063758464 @default.
- W2058967072 cites W2072629032 @default.
- W2058967072 cites W2088517066 @default.
- W2058967072 cites W2099660384 @default.
- W2058967072 cites W2110221887 @default.
- W2058967072 cites W2116845203 @default.
- W2058967072 cites W2129317658 @default.
- W2058967072 cites W2152531639 @default.
- W2058967072 doi "https://doi.org/10.1016/s0378-1119(96)00388-5" @default.
- W2058967072 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8973339" @default.
- W2058967072 hasPublicationYear "1996" @default.
- W2058967072 type Work @default.
- W2058967072 sameAs 2058967072 @default.
- W2058967072 citedByCount "60" @default.
- W2058967072 countsByYear W20589670722012 @default.
- W2058967072 countsByYear W20589670722013 @default.
- W2058967072 countsByYear W20589670722014 @default.
- W2058967072 countsByYear W20589670722015 @default.
- W2058967072 countsByYear W20589670722016 @default.
- W2058967072 countsByYear W20589670722017 @default.
- W2058967072 countsByYear W20589670722018 @default.
- W2058967072 countsByYear W20589670722021 @default.
- W2058967072 countsByYear W20589670722023 @default.
- W2058967072 crossrefType "journal-article" @default.
- W2058967072 hasAuthorship W2058967072A5044292989 @default.
- W2058967072 hasAuthorship W2058967072A5070917422 @default.
- W2058967072 hasAuthorship W2058967072A5076921040 @default.
- W2058967072 hasConcept C104317684 @default.
- W2058967072 hasConcept C105580179 @default.
- W2058967072 hasConcept C149364088 @default.
- W2058967072 hasConcept C150194340 @default.
- W2058967072 hasConcept C153911025 @default.
- W2058967072 hasConcept C159047783 @default.
- W2058967072 hasConcept C159654299 @default.
- W2058967072 hasConcept C195616568 @default.
- W2058967072 hasConcept C202878990 @default.
- W2058967072 hasConcept C2777576037 @default.
- W2058967072 hasConcept C2779222958 @default.
- W2058967072 hasConcept C54355233 @default.
- W2058967072 hasConcept C86803240 @default.
- W2058967072 hasConceptScore W2058967072C104317684 @default.
- W2058967072 hasConceptScore W2058967072C105580179 @default.
- W2058967072 hasConceptScore W2058967072C149364088 @default.
- W2058967072 hasConceptScore W2058967072C150194340 @default.
- W2058967072 hasConceptScore W2058967072C153911025 @default.
- W2058967072 hasConceptScore W2058967072C159047783 @default.
- W2058967072 hasConceptScore W2058967072C159654299 @default.
- W2058967072 hasConceptScore W2058967072C195616568 @default.
- W2058967072 hasConceptScore W2058967072C202878990 @default.
- W2058967072 hasConceptScore W2058967072C2777576037 @default.
- W2058967072 hasConceptScore W2058967072C2779222958 @default.
- W2058967072 hasConceptScore W2058967072C54355233 @default.
- W2058967072 hasConceptScore W2058967072C86803240 @default.
- W2058967072 hasIssue "1-2" @default.
- W2058967072 hasLocation W20589670721 @default.
- W2058967072 hasLocation W20589670722 @default.
- W2058967072 hasOpenAccess W2058967072 @default.
- W2058967072 hasPrimaryLocation W20589670721 @default.
- W2058967072 hasRelatedWork W2054129679 @default.
- W2058967072 hasRelatedWork W2056830595 @default.
- W2058967072 hasRelatedWork W2063265750 @default.
- W2058967072 hasRelatedWork W2085675226 @default.
- W2058967072 hasRelatedWork W2087367099 @default.
- W2058967072 hasRelatedWork W2087461897 @default.
- W2058967072 hasRelatedWork W2107865312 @default.
- W2058967072 hasRelatedWork W2188478563 @default.
- W2058967072 hasRelatedWork W2343667559 @default.
- W2058967072 hasRelatedWork W2935912945 @default.
- W2058967072 hasVolume "180" @default.
- W2058967072 isParatext "false" @default.
- W2058967072 isRetracted "false" @default.
- W2058967072 magId "2058967072" @default.
- W2058967072 workType "article" @default.