FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2058992909> ?p ?o ?g. }

• W2058992909 endingPage "889" @default.
• W2058992909 startingPage "877" @default.
• W2058992909 abstract "The viscosity of bovine liver glutamate dehydrogenase solutions was studied at 10 and 20° C in 0.2.M sodium phosphate buffer at pH 7, in the concentration range 0.1–8 mg/ml. A method for the study of the viscosity of very dilute solutions of associating enzymes is described. It was found that the reduced specific viscosity ηsp/c of glutamate dehydrogenase continuously increases with increasing enzyme concentration, from about 4 ml/g at the lowest concentrations to about 16 ml/g at 8 mg/ml. In the presence of 10−3M GTP and 10−3M NADH the viscosity increase is much smaller and the results can be extrapolated to zero enzyme concentration to yield an intrinsic viscosity [η] = 3.2. The values of ηsp/c in phosphate buffer alone apparently extrapolate to the same value of [η], or to a value close to it. We also observe that, in the presence of toluene ηsp/c increases very much more with enzyme concentration: ηsp/c already equals 16 ml/g at a concentration of 0.75 mg/ml. These observations are in good agreement with the hypothesis that the active oligomer of glutamate dehydrogenase (MW = 312,000) associates with increasing enzyme concentration to form linear rodlike polymers of indefinite length. This association is strongly diminished by the addition of 10−3M GTP, 10−3M NADH. Toluene, on the other hand, promotes reversible association to linear polymers of very high molecular weight. The transverse and axial rotary frictional coefficients of macroscopic bodies, similar to a physical model for the structure of glutamate dehydrogenase recently advanced, were determined. Assuming that the viscosity of the model is equal to that of an ellipsoid of rotation with identical frictional coefficients, we calculate [η] = 3.26 ml/g according to Kuhn and 3.20 ml/g according to Simha, for the glutamate dehydrogenase oligomer, in good agreement with the result derived from the study of enzyme solutions." @default.
• W2058992909 created "2016-06-24" @default.
• W2058992909 creator A5036922345 @default.
• W2058992909 creator A5038398169 @default.
• W2058992909 date "1970-08-01" @default.
• W2058992909 modified "2023-10-16" @default.
• W2058992909 title "Studies on the viscosity of solutions of bovine liver glutamate dehydrogenase and on related hydrodynamic models; effect of toluene on enzyme association" @default.
• W2058992909 cites W1017217751 @default.
• W2058992909 cites W1552245034 @default.
• W2058992909 cites W1982186104 @default.
• W2058992909 cites W1996636548 @default.
• W2058992909 cites W2003525044 @default.
• W2058992909 cites W2008690167 @default.
• W2058992909 cites W2009972793 @default.
• W2058992909 cites W2017275800 @default.
• W2058992909 cites W2020031152 @default.
• W2058992909 cites W2025304722 @default.
• W2058992909 cites W2032384898 @default.
• W2058992909 cites W2041267309 @default.
• W2058992909 cites W2056437971 @default.
• W2058992909 cites W2058419806 @default.
• W2058992909 cites W2059862595 @default.
• W2058992909 cites W2071994094 @default.
• W2058992909 cites W2121882775 @default.
• W2058992909 cites W2248358076 @default.
• W2058992909 cites W2314502305 @default.
• W2058992909 cites W2318354357 @default.
• W2058992909 cites W2331862502 @default.
• W2058992909 doi "https://doi.org/10.1002/bip.1970.360090802" @default.
• W2058992909 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/5433614" @default.
• W2058992909 hasPublicationYear "1970" @default.
• W2058992909 type Work @default.
• W2058992909 sameAs 2058992909 @default.
• W2058992909 citedByCount "47" @default.
• W2058992909 countsByYear W20589929092013 @default.
• W2058992909 countsByYear W20589929092016 @default.
• W2058992909 crossrefType "journal-article" @default.
• W2058992909 hasAuthorship W2058992909A5036922345 @default.
• W2058992909 hasAuthorship W2058992909A5038398169 @default.
• W2058992909 hasConcept C121332964 @default.
• W2058992909 hasConcept C127172972 @default.
• W2058992909 hasConcept C170493617 @default.
• W2058992909 hasConcept C178790620 @default.
• W2058992909 hasConcept C181199279 @default.
• W2058992909 hasConcept C185592680 @default.
• W2058992909 hasConcept C26360006 @default.
• W2058992909 hasConcept C2776317432 @default.
• W2058992909 hasConcept C2777202666 @default.
• W2058992909 hasConcept C2778142098 @default.
• W2058992909 hasConcept C43617362 @default.
• W2058992909 hasConcept C521977710 @default.
• W2058992909 hasConcept C55493867 @default.
• W2058992909 hasConcept C61174792 @default.
• W2058992909 hasConcept C97355855 @default.
• W2058992909 hasConceptScore W2058992909C121332964 @default.
• W2058992909 hasConceptScore W2058992909C127172972 @default.
• W2058992909 hasConceptScore W2058992909C170493617 @default.
• W2058992909 hasConceptScore W2058992909C178790620 @default.
• W2058992909 hasConceptScore W2058992909C181199279 @default.
• W2058992909 hasConceptScore W2058992909C185592680 @default.
• W2058992909 hasConceptScore W2058992909C26360006 @default.
• W2058992909 hasConceptScore W2058992909C2776317432 @default.
• W2058992909 hasConceptScore W2058992909C2777202666 @default.
• W2058992909 hasConceptScore W2058992909C2778142098 @default.
• W2058992909 hasConceptScore W2058992909C43617362 @default.
• W2058992909 hasConceptScore W2058992909C521977710 @default.
• W2058992909 hasConceptScore W2058992909C55493867 @default.
• W2058992909 hasConceptScore W2058992909C61174792 @default.
• W2058992909 hasConceptScore W2058992909C97355855 @default.
• W2058992909 hasIssue "8" @default.
• W2058992909 hasLocation W20589929091 @default.
• W2058992909 hasLocation W20589929092 @default.
• W2058992909 hasOpenAccess W2058992909 @default.
• W2058992909 hasPrimaryLocation W20589929091 @default.
• W2058992909 hasRelatedWork W1503945197 @default.
• W2058992909 hasRelatedWork W178112583 @default.
• W2058992909 hasRelatedWork W2039192497 @default.
• W2058992909 hasRelatedWork W2075849912 @default.
• W2058992909 hasRelatedWork W2096786065 @default.
• W2058992909 hasRelatedWork W2110803271 @default.
• W2058992909 hasRelatedWork W2136225779 @default.
• W2058992909 hasRelatedWork W2469339984 @default.
• W2058992909 hasRelatedWork W91120528 @default.
• W2058992909 hasRelatedWork W2181583054 @default.
• W2058992909 hasVolume "9" @default.
• W2058992909 isParatext "false" @default.
• W2058992909 isRetracted "false" @default.
• W2058992909 magId "2058992909" @default.
• W2058992909 workType "article" @default.