FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2059011987> ?p ?o ?g. }

• W2059011987 endingPage "1838" @default.
• W2059011987 startingPage "1826" @default.
• W2059011987 abstract "The fundamental myocellular uptake and retention mechanisms of hexakis (2-methoxyisobutyl isonitrile) technetium(I) (Tc-MIBI), a technetium-99m-based myocardial perfusion imaging agent, are unresolved. Because of the lipophilic cationic nature of Tc-MIBI, it may be distributed across biological membranes in response to transmembrane potential. To test this hypothesis, net uptake and retention of Tc-MIBI in cultured chick embryo ventricular myocytes were determined under conditions known to alter mitochondrial and plasma membrane potentials. Isovolumic depolarization of plasma membrane potentials in 130 mM extracellular K (Ko) 20 mM extracellular Cl buffer reduced net accumulation of Tc-MIBI from 171 +/- 16 (control) to 29 +/- 3.3 fmol intracellular Tc-MIBI/mg protein.nM extracellular Tc-MIBI. Unidirectional influx of Tc-MIBI in cells depolarized in 30 mM Ko buffer was also reduced; a resting plasma membrane potential of -87 +/- 6 mV was calculated from the Goldman flux equation using normal Ko/high Ko Tc-MIBI influx ratios. Addition of the potassium ionophore valinomycin to cells incubated in 130 mM Ko buffer to additionally depolarize mitochondrial membrane potentials further reduced net uptake of Tc-MIBI to levels comparable to that found in nonviable freeze-thawed preparations ([Tc-MIBI]i/[Tc-MIBI]o = 1). By depolarizing mitochondrial (and in part plasma membrane) potentials with the protonophores 2,4-dinitrophenol and carbonyl cyanide m-chlorophenylhydrazone (CCCP) Tc-MIBI was rapidly depleted from 181 +/- 16 (control) to 16 +/- 2.6 and 31 +/- 4.2 fmol/mg protein.nMo, respectively, with kinetics that did not correlate with loss of cellular ATP content. CCCP alone inhibited 90 +/- 3% of net accumulation or 66 +/- 3% of unidirectional influx of Tc-MIBI in a concentration-dependent manner. By hyperpolarizing mitochondrial membrane potentials with the K+/H+ ionophore nigericin or the ATP synthase inhibitor oligomycin, net uptake and retention of Tc-MIBI were increased by 60 +/- 9% and 375 +/- 20%, respectively. Caffeine, as well as the respiratory chain electron transport inhibitor rotenone, did not significantly alter net cell uptake (p greater than 0.2). These data indicate that the fundamental myocellular uptake mechanism of Tc-MIBI involves passive distribution across plasma and mitochondrial membranes and that at equilibrium Tc-MIBI is sequestered within mitochondria by the large negative transmembrane potentials." @default.
• W2059011987 created "2016-06-24" @default.
• W2059011987 creator A5047326561 @default.
• W2059011987 creator A5051752608 @default.
• W2059011987 creator A5068852591 @default.
• W2059011987 date "1990-11-01" @default.
• W2059011987 modified "2023-10-17" @default.
• W2059011987 title "Uptake and retention of hexakis (2-methoxyisobutyl isonitrile) technetium(I) in cultured chick myocardial cells. Mitochondrial and plasma membrane potential dependence." @default.
• W2059011987 cites W1495376651 @default.
• W2059011987 cites W1504836130 @default.
• W2059011987 cites W1553956082 @default.
• W2059011987 cites W1594597809 @default.
• W2059011987 cites W1605717451 @default.
• W2059011987 cites W164548000 @default.
• W2059011987 cites W1775749144 @default.
• W2059011987 cites W1971706169 @default.
• W2059011987 cites W1986997254 @default.
• W2059011987 cites W1993062133 @default.
• W2059011987 cites W1998172061 @default.
• W2059011987 cites W1998359737 @default.
• W2059011987 cites W2001011278 @default.
• W2059011987 cites W2002413733 @default.
• W2059011987 cites W2008117638 @default.
• W2059011987 cites W2029864607 @default.
• W2059011987 cites W2033256212 @default.
• W2059011987 cites W2034223710 @default.
• W2059011987 cites W2049911211 @default.
• W2059011987 cites W2062371642 @default.
• W2059011987 cites W2067877174 @default.
• W2059011987 cites W2070994754 @default.
• W2059011987 cites W2090751202 @default.
• W2059011987 cites W2096012295 @default.
• W2059011987 cites W2096298364 @default.
• W2059011987 cites W2105979237 @default.
• W2059011987 cites W2114388515 @default.
• W2059011987 cites W2118340418 @default.
• W2059011987 cites W2133764164 @default.
• W2059011987 cites W2145107737 @default.
• W2059011987 cites W2156441977 @default.
• W2059011987 cites W2181814457 @default.
• W2059011987 cites W2340884455 @default.
• W2059011987 cites W2345867216 @default.
• W2059011987 cites W2409363906 @default.
• W2059011987 cites W2417835997 @default.
• W2059011987 cites W2418558666 @default.
• W2059011987 cites W2886302980 @default.
• W2059011987 doi "https://doi.org/10.1161/01.cir.82.5.1826" @default.
• W2059011987 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2225379" @default.
• W2059011987 hasPublicationYear "1990" @default.
• W2059011987 type Work @default.
• W2059011987 sameAs 2059011987 @default.
• W2059011987 citedByCount "430" @default.
• W2059011987 countsByYear W20590119872012 @default.
• W2059011987 countsByYear W20590119872013 @default.
• W2059011987 countsByYear W20590119872014 @default.
• W2059011987 countsByYear W20590119872015 @default.
• W2059011987 countsByYear W20590119872016 @default.
• W2059011987 countsByYear W20590119872017 @default.
• W2059011987 countsByYear W20590119872018 @default.
• W2059011987 countsByYear W20590119872019 @default.
• W2059011987 countsByYear W20590119872020 @default.
• W2059011987 countsByYear W20590119872021 @default.
• W2059011987 countsByYear W20590119872022 @default.
• W2059011987 countsByYear W20590119872023 @default.
• W2059011987 crossrefType "journal-article" @default.
• W2059011987 hasAuthorship W2059011987A5047326561 @default.
• W2059011987 hasAuthorship W2059011987A5051752608 @default.
• W2059011987 hasAuthorship W2059011987A5068852591 @default.
• W2059011987 hasBestOaLocation W20590119871 @default.
• W2059011987 hasConcept C12554922 @default.
• W2059011987 hasConcept C181911157 @default.
• W2059011987 hasConcept C185592680 @default.
• W2059011987 hasConcept C2778385715 @default.
• W2059011987 hasConcept C2778421351 @default.
• W2059011987 hasConcept C2779014961 @default.
• W2059011987 hasConcept C28406088 @default.
• W2059011987 hasConcept C4141045 @default.
• W2059011987 hasConcept C41625074 @default.
• W2059011987 hasConcept C55493867 @default.
• W2059011987 hasConcept C79879829 @default.
• W2059011987 hasConcept C86803240 @default.
• W2059011987 hasConceptScore W2059011987C12554922 @default.
• W2059011987 hasConceptScore W2059011987C181911157 @default.
• W2059011987 hasConceptScore W2059011987C185592680 @default.
• W2059011987 hasConceptScore W2059011987C2778385715 @default.
• W2059011987 hasConceptScore W2059011987C2778421351 @default.
• W2059011987 hasConceptScore W2059011987C2779014961 @default.
• W2059011987 hasConceptScore W2059011987C28406088 @default.
• W2059011987 hasConceptScore W2059011987C4141045 @default.
• W2059011987 hasConceptScore W2059011987C41625074 @default.
• W2059011987 hasConceptScore W2059011987C55493867 @default.
• W2059011987 hasConceptScore W2059011987C79879829 @default.
• W2059011987 hasConceptScore W2059011987C86803240 @default.
• W2059011987 hasIssue "5" @default.
• W2059011987 hasLocation W20590119871 @default.
• W2059011987 hasLocation W20590119872 @default.
• W2059011987 hasOpenAccess W2059011987 @default.
• W2059011987 hasPrimaryLocation W20590119871 @default.

• next