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- W2059053178 abstract "Monoglyceride lipase (MGL) has been produced with the baculovirus-insect cell system. The mouse MGL cDNA was subcloned into a baculovirus transfer vector in frame with a sequence encoding an N-terminal stretch of six histidine residues. Purification to apparent homogeneity was obtained by nickel-chelating chromatography. The final yield was 3 mg of pure enzymatically active MGL per liter of Sf9 cell suspension culture. Analysis by SDS-PAGE and mass spectrometry showed that the recombinant histidine-tagged enzyme had the expected molecular mass. With monoolein as substrate, the specific activity and the apparent K(m) were close to those of rat MGL of adipose tissue." @default.
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- W2059053178 date "2000-04-01" @default.
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- W2059053178 title "Expression, Purification, and Characterization of Histidine-Tagged Mouse Monoglyceride Lipase from Baculovirus-Infected Insect Cells" @default.
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- W2059053178 doi "https://doi.org/10.1006/prep.1999.1194" @default.
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