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- W2059092257 abstract "We have developed an enzyme-linked immunosorbent assay for the quantification of turbot (Scophthalmus maximus) immunoglobulin (Ig). The capture antibody is a rabbit polyclonal antiserum to turbot Ig, and the detector antibody a monoclonal antibody (UR3) to the turbot Ig heavy chain. Both antibodies bind nearly 100% of turbot Ig. The assay allows detection of turbot Ig in serum at concentrations as low as 0.16 μg ml−1 and takes less than 4 h. Precision is satisfactory, with intra-assay coefficients of variation (CVs) ranging from 2.1 to 16.6%, and inter-assay CVs ranging from 5.8 to 24.6%. We used the assay to determine Ig concentrations in the sera of healthy turbot of different weights. Mean serum Ig concentration was 3.35 ± 0.74 mg ml−1 for fish weighing 15–25 g and 11.14 ± 1.87 mg ml−1 for fish weighing 1000–2000 g." @default.
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- W2059092257 date "1995-03-01" @default.
- W2059092257 modified "2023-10-17" @default.
- W2059092257 title "A sandwich immunoassay to quantify low levels of turbot (Scophthalmus maximus) immunoglobulins" @default.
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- W2059092257 doi "https://doi.org/10.1016/0165-2427(94)05339-t" @default.
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