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- W2059096070 abstract "Alginate has been used as a gelling agent for culture medium in which tobacco and petunia protoplasts were suspended. The gel, formed by cation dependent cross-linkages, supported colony formation with an efficiency comparable to agar medium. The alginate matrix was liquefied by addition of a chelating agent, making possible the recovery of developing colonies without significantly affecting their continued growth. The use of this plating technique offers not only the advantage of suspending plant material in semi-solid medium without temperature shock but also the potential for subsequent large scale recovery." @default.
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- W2059096070 date "1982-04-01" @default.
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- W2059096070 title "Alginate: An alternative to agar in plant protoplast culture" @default.
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- W2059096070 doi "https://doi.org/10.1016/0304-4211(82)90207-3" @default.
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