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• W2059110319 abstract "Mitotic spindles were isolated from Chinese hamster ovary cells with an isolation medium that contained 5% glycerol, 1% dimethyl sulfoxide, 5% polyethylene glycol, 5 mM piperazine-N, N'-bis(2-ethanesulfonic acid), 1 mM ethylene glycol bis(β-aminoethylether)tetraacetic acid, 0.5 mM MgSO4, 0.5% Triton X-100, 0.5 mM GTP and 0.5 mM dithiothreitol at pH 6.8. The chromosomes generally were lost from the preparation; thus, electron microscopy of whole-mount preparations showed isolated spindles with a microtubule array in the spindle that attached to the centrosomes at each pole.An increase in the concentration of MgSO4 to 5 mM stabilized the chromosomal structure, and mitotic spindles with chromosomes attached in almost the same configuration as in vivo could be isolated.A general discussion of the procedures for isolating mitotic spindles from tissue cultured cells is included." @default.
• W2059110319 created "2016-06-24" @default.
• W2059110319 creator A5078916258 @default.
• W2059110319 date "1982-01-01" @default.
• W2059110319 modified "2023-10-16" @default.
• W2059110319 title "Isolation of mitotic spindles from Chinese hamster ovary cells." @default.
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• W2059110319 doi "https://doi.org/10.1247/csf.7.307" @default.
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