Matches in SemOpenAlex for { <https://semopenalex.org/work/W2059129047> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2059129047 abstract "Naturally occurring suppressor mutants derived from tRNATrp genes have never been identified in S. cerevisiae. Oligonucleotide-directed mutagenesis was used to generate potential ochre and opal suppressors from a cloned tRNATrp gene. In vitro transcription analyses show the ochre suppressor form of the gene, TRPO, accumulates precursors and tRNA in amounts comparable to the parent. The opal suppressor, TRPOP, accumulates 4-5 fold less tRNA. Both forms of the gene are processed and spliced in vitro to produce tRNAs with the expected base sequences. The altered genes were subcloned into yeast vectors and introduced into yeast strains carrying a variety of amber, ochre, and opal mutations. When introduced on a CEN vector, neither ochre nor opal suppressor forms show suppressor activity. Deletion of the CEN region from the clones increases the copy number to 10-20/cell. The opal suppressor form shows moderate suppressor activity when the gene is introduced on this vector, however, the ochre suppressor form exhibits no detectable biological activity regardless of gene copy number. Northern blot analyses of the steady state levels of tRNATrp in cells containing the high copy-number clones reveal 20-100% increases in the abundance of tRNATrp." @default.
- W2059129047 created "2016-06-24" @default.
- W2059129047 creator A5018124229 @default.
- W2059129047 creator A5023851727 @default.
- W2059129047 creator A5050242902 @default.
- W2059129047 creator A5065291551 @default.
- W2059129047 creator A5080055434 @default.
- W2059129047 date "1990-01-01" @default.
- W2059129047 modified "2023-09-27" @default.
- W2059129047 title "Yeast tRNATrpgenes with anticodons corresponding to UAA and UGA nonsense codons" @default.
- W2059129047 cites W114751351 @default.
- W2059129047 cites W1534929651 @default.
- W2059129047 cites W1762323640 @default.
- W2059129047 cites W1925521833 @default.
- W2059129047 cites W1970719040 @default.
- W2059129047 cites W1972438172 @default.
- W2059129047 cites W1973782344 @default.
- W2059129047 cites W1982165710 @default.
- W2059129047 cites W1988947691 @default.
- W2059129047 cites W1990159369 @default.
- W2059129047 cites W2001870577 @default.
- W2059129047 cites W2003372674 @default.
- W2059129047 cites W2005851116 @default.
- W2059129047 cites W2012920273 @default.
- W2059129047 cites W2017348860 @default.
- W2059129047 cites W2018316824 @default.
- W2059129047 cites W2019410656 @default.
- W2059129047 cites W2029377254 @default.
- W2059129047 cites W2038115521 @default.
- W2059129047 cites W2051029292 @default.
- W2059129047 cites W2089428413 @default.
- W2059129047 cites W2091836061 @default.
- W2059129047 cites W2122217480 @default.
- W2059129047 cites W2132417094 @default.
- W2059129047 cites W2316071335 @default.
- W2059129047 cites W2411330586 @default.
- W2059129047 cites W2418469048 @default.
- W2059129047 doi "https://doi.org/10.1093/nar/18.14.4215" @default.
- W2059129047 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/331181" @default.
- W2059129047 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2198538" @default.
- W2059129047 hasPublicationYear "1990" @default.
- W2059129047 type Work @default.
- W2059129047 sameAs 2059129047 @default.
- W2059129047 citedByCount "13" @default.
- W2059129047 countsByYear W20591290472012 @default.
- W2059129047 countsByYear W20591290472013 @default.
- W2059129047 crossrefType "journal-article" @default.
- W2059129047 hasAuthorship W2059129047A5018124229 @default.
- W2059129047 hasAuthorship W2059129047A5023851727 @default.
- W2059129047 hasAuthorship W2059129047A5050242902 @default.
- W2059129047 hasAuthorship W2059129047A5065291551 @default.
- W2059129047 hasAuthorship W2059129047A5080055434 @default.
- W2059129047 hasBestOaLocation W20591290472 @default.
- W2059129047 hasConcept C104317684 @default.
- W2059129047 hasConcept C143065580 @default.
- W2059129047 hasConcept C153911025 @default.
- W2059129047 hasConcept C153957851 @default.
- W2059129047 hasConcept C179185449 @default.
- W2059129047 hasConcept C2777576037 @default.
- W2059129047 hasConcept C54355233 @default.
- W2059129047 hasConcept C67705224 @default.
- W2059129047 hasConcept C86803240 @default.
- W2059129047 hasConceptScore W2059129047C104317684 @default.
- W2059129047 hasConceptScore W2059129047C143065580 @default.
- W2059129047 hasConceptScore W2059129047C153911025 @default.
- W2059129047 hasConceptScore W2059129047C153957851 @default.
- W2059129047 hasConceptScore W2059129047C179185449 @default.
- W2059129047 hasConceptScore W2059129047C2777576037 @default.
- W2059129047 hasConceptScore W2059129047C54355233 @default.
- W2059129047 hasConceptScore W2059129047C67705224 @default.
- W2059129047 hasConceptScore W2059129047C86803240 @default.
- W2059129047 hasLocation W20591290471 @default.
- W2059129047 hasLocation W20591290472 @default.
- W2059129047 hasLocation W20591290473 @default.
- W2059129047 hasOpenAccess W2059129047 @default.
- W2059129047 hasPrimaryLocation W20591290471 @default.
- W2059129047 hasRelatedWork W1528095108 @default.
- W2059129047 hasRelatedWork W1968643238 @default.
- W2059129047 hasRelatedWork W1974992467 @default.
- W2059129047 hasRelatedWork W1982942059 @default.
- W2059129047 hasRelatedWork W1992205301 @default.
- W2059129047 hasRelatedWork W2021380639 @default.
- W2059129047 hasRelatedWork W2076407061 @default.
- W2059129047 hasRelatedWork W2111958127 @default.
- W2059129047 hasRelatedWork W2320378423 @default.
- W2059129047 hasRelatedWork W2324532374 @default.
- W2059129047 isParatext "false" @default.
- W2059129047 isRetracted "false" @default.
- W2059129047 magId "2059129047" @default.
- W2059129047 workType "article" @default.