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• W2059173724 endingPage "458" @default.
• W2059173724 startingPage "448" @default.
• W2059173724 abstract "The assessment of genotoxic potential in surface water requires test methods, among which are those that detect initial DNA damage in organisms of aquatic biocenosis. The microgel electrophoresis (MGE) “comet assay” was applied to a ubiquitous unicellular green alga (Chlamydomonas reinhardtii) to detect DNA damage caused by genotoxins. For this, the test protocol described by Singh NP et al. [Exp Cell Res 175:184–191, 1988] was modified. Major modifications were the use of alkaline lysis buffer with ionic detergents and the reduction of preincubation and electrophoresis times. Short-time exposure of Chlamydomonas to the well-known genotoxicants 4-nitroquinoline-1-oxide (4-NQO), N-nitrosodimethylamine, and hydrogen peroxide led to dose-dependent DNA damage. Chlamydomonas responded very sensitively to treatment with increasing doses of 4-NQO. At a concentration of 25 nM, significant DNA damage was observed. At higher 4-NQO doses (>100 nM), DNA damage was visible as complete DNA fragmentation into fine granules. N-Nitrosodimethylamine caused genotoxic effects at a concentration range from 0.014 to 0.14 mM without producing complete DNA fragmentation at the concentrations tested (highest dose, 140 mM). To evaluate the influence of illumination conditions during exposure, cells were incubated with increasing doses of H2O2 (0.25–1.0 mM) in darkness and in light. Our results indicate that incubation in light enables Chlamydomonas to cope with oxidative stress more efficiently than under dark conditions. To a certain extent, cytotoxic as well as genotoxic effects of H2O2 depend on the illumination condition or repair and anti-oxidative protection mechanisms activated by light, respectively. Environ. Mol. Mutagen. 30:448–458, 1997 © 1997 Wiley-Liss, Inc." @default.
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• W2059173724 date "1997-01-01" @default.
• W2059173724 modified "2023-09-30" @default.
• W2059173724 title "Detection of primary DNA damage inChlamydomonas reinhardtii by means of modified microgel electrophoresis" @default.
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• W2059173724 doi "https://doi.org/10.1002/(sici)1098-2280(1997)30:4<448::aid-em10>3.0.co;2-d" @default.
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