Matches in SemOpenAlex for { <https://semopenalex.org/work/W2059185823> ?p ?o ?g. }
- W2059185823 endingPage "484" @default.
- W2059185823 startingPage "479" @default.
- W2059185823 abstract "Abstract An improved headspace capillary gas chromatographic (GC) method was developed to measure the oxidative susceptibility of human red blood cell (RBC) membranes. This method analyzed volatile peroxidation products of both n−6 (hexanal and pentane) and n−3 (propanal) polyunsaturated fatty acids. Oxidative susceptibility tests were standardized by incubating in a sealed 10‐mL headspace bottle 0.25 or 1 mL of human RBC membrane in 40 mM phosphate buffer for 1 hr at 37°C with a mixture of Fe ++ , ascorbic acid and H 2 O 2 . Sodium dodecyl sulfate increased significantly the amount of hexanal measured by headspace GC. By this standard headspace method, in one series of red blood cell membranes (RBCM) samples a four‐fold variation in oxidative susceptibility was observed in RBCM from blood freshly drawn from six healthy subjects. In another series of RBCM samples a sixteen‐fold variation in oxidative susceptibility was noted in frozen RBCM from blood freshly drawn from five healthy subjects. Correlation between hexanal formation and polyunsaturated fatty acids (PUFA) depletion provided good evidence that under these standard conditions hexanal is exclusively derived from the oxidation of arachidonic acid. Hydroperoxides of arachidonic acid are more readily formed and decomposed than those of linoleic acid in the presence of Fe ++ , ascorbic acid and H 2 O 2 to produce hexanal as the main product that can be readily analyzed by headspace GC. This method may provide a useful tool to study susceptibility toward lipid peroxidative damage in human RBC membranes." @default.
- W2059185823 created "2016-06-24" @default.
- W2059185823 creator A5000331684 @default.
- W2059185823 creator A5016356461 @default.
- W2059185823 date "1991-06-01" @default.
- W2059185823 modified "2023-10-14" @default.
- W2059185823 title "Headspace gas chromatography of volatile lipid peroxidation products from human red blood cell membranes" @default.
- W2059185823 cites W1531656041 @default.
- W2059185823 cites W1576995966 @default.
- W2059185823 cites W1602505196 @default.
- W2059185823 cites W1734082729 @default.
- W2059185823 cites W1758496201 @default.
- W2059185823 cites W1964015231 @default.
- W2059185823 cites W1969895637 @default.
- W2059185823 cites W1979413960 @default.
- W2059185823 cites W1988445201 @default.
- W2059185823 cites W1997299719 @default.
- W2059185823 cites W2015257597 @default.
- W2059185823 cites W2016656152 @default.
- W2059185823 cites W2018082171 @default.
- W2059185823 cites W2019449116 @default.
- W2059185823 cites W2031067130 @default.
- W2059185823 cites W2032508526 @default.
- W2059185823 cites W2034467957 @default.
- W2059185823 cites W2035591072 @default.
- W2059185823 cites W2035809899 @default.
- W2059185823 cites W2039108603 @default.
- W2059185823 cites W2045309313 @default.
- W2059185823 cites W2051174549 @default.
- W2059185823 cites W2054680647 @default.
- W2059185823 cites W2055983834 @default.
- W2059185823 cites W2060319698 @default.
- W2059185823 cites W2073809775 @default.
- W2059185823 cites W2114760606 @default.
- W2059185823 cites W2115303076 @default.
- W2059185823 cites W2128635872 @default.
- W2059185823 cites W2132137034 @default.
- W2059185823 cites W2211467290 @default.
- W2059185823 cites W2262246957 @default.
- W2059185823 cites W2406835475 @default.
- W2059185823 cites W2416349776 @default.
- W2059185823 cites W2417616727 @default.
- W2059185823 cites W2419654827 @default.
- W2059185823 cites W4293247451 @default.
- W2059185823 cites W69161556 @default.
- W2059185823 cites W969915667 @default.
- W2059185823 doi "https://doi.org/10.1007/bf02536076" @default.
- W2059185823 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1908930" @default.
- W2059185823 hasPublicationYear "1991" @default.
- W2059185823 type Work @default.
- W2059185823 sameAs 2059185823 @default.
- W2059185823 citedByCount "42" @default.
- W2059185823 countsByYear W20591858232015 @default.
- W2059185823 countsByYear W20591858232017 @default.
- W2059185823 countsByYear W20591858232020 @default.
- W2059185823 countsByYear W20591858232021 @default.
- W2059185823 countsByYear W20591858232023 @default.
- W2059185823 crossrefType "journal-article" @default.
- W2059185823 hasAuthorship W2059185823A5000331684 @default.
- W2059185823 hasAuthorship W2059185823A5016356461 @default.
- W2059185823 hasConcept C181199279 @default.
- W2059185823 hasConcept C185592680 @default.
- W2059185823 hasConcept C19038510 @default.
- W2059185823 hasConcept C2776557347 @default.
- W2059185823 hasConcept C2777551382 @default.
- W2059185823 hasConcept C2778004101 @default.
- W2059185823 hasConcept C2778078955 @default.
- W2059185823 hasConcept C2778353584 @default.
- W2059185823 hasConcept C2780809830 @default.
- W2059185823 hasConcept C2780829032 @default.
- W2059185823 hasConcept C2986274086 @default.
- W2059185823 hasConcept C31903555 @default.
- W2059185823 hasConcept C41625074 @default.
- W2059185823 hasConcept C43617362 @default.
- W2059185823 hasConcept C543025807 @default.
- W2059185823 hasConcept C55493867 @default.
- W2059185823 hasConceptScore W2059185823C181199279 @default.
- W2059185823 hasConceptScore W2059185823C185592680 @default.
- W2059185823 hasConceptScore W2059185823C19038510 @default.
- W2059185823 hasConceptScore W2059185823C2776557347 @default.
- W2059185823 hasConceptScore W2059185823C2777551382 @default.
- W2059185823 hasConceptScore W2059185823C2778004101 @default.
- W2059185823 hasConceptScore W2059185823C2778078955 @default.
- W2059185823 hasConceptScore W2059185823C2778353584 @default.
- W2059185823 hasConceptScore W2059185823C2780809830 @default.
- W2059185823 hasConceptScore W2059185823C2780829032 @default.
- W2059185823 hasConceptScore W2059185823C2986274086 @default.
- W2059185823 hasConceptScore W2059185823C31903555 @default.
- W2059185823 hasConceptScore W2059185823C41625074 @default.
- W2059185823 hasConceptScore W2059185823C43617362 @default.
- W2059185823 hasConceptScore W2059185823C543025807 @default.
- W2059185823 hasConceptScore W2059185823C55493867 @default.
- W2059185823 hasIssue "6" @default.
- W2059185823 hasLocation W20591858231 @default.
- W2059185823 hasLocation W20591858232 @default.
- W2059185823 hasOpenAccess W2059185823 @default.
- W2059185823 hasPrimaryLocation W20591858231 @default.
- W2059185823 hasRelatedWork W1496885974 @default.