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- W2059187839 abstract "Transgenesis is a cornerstone of molecular biology. The ability to integrate a specifically engineered piece of DNA into the genome of a living system is fundamental to our efforts to understand life and exploit its implications for medicine, nanotechnology and bioprospecting. However, transgenesis has been hampered by position effects and multi-copy integration problems, which are mainly due to the use of small, plasmid-based transgenes. Large transgenes based on native genomic regions cloned into bacterial artificial chromosomes (BACs) circumvent these problems but are prone to fragmentation. Herein, we report that contrary to widely held notions, large BAC-sized constructs do not prohibit transposition. We also report the first reliable method for BAC transgenesis in human embryonic stem cells (hESCs). The PiggyBac or Sleeping Beauty transposon inverted repeats were integrated into BAC vectors by recombineering, followed by co-lipofection with the corresponding transposase in hESCs to generate robust fluorescent protein reporter lines for OCT4, NANOG, GATA4 and PAX6. BAC transposition delivers several advantages, including increased frequencies of single-copy, full-length integration, which will be useful in all transgenic systems but especially in difficult venues like hESCs." @default.
- W2059187839 created "2016-06-24" @default.
- W2059187839 creator A5002051588 @default.
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- W2059187839 creator A5087616533 @default.
- W2059187839 date "2012-06-30" @default.
- W2059187839 modified "2023-10-14" @default.
- W2059187839 title "Transposon-mediated BAC transgenesis in human ES cells" @default.
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- W2059187839 cites W1850310634 @default.
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- W2059187839 cites W1977353721 @default.
- W2059187839 cites W1977416347 @default.
- W2059187839 cites W1982179068 @default.
- W2059187839 cites W1982784580 @default.
- W2059187839 cites W1983543025 @default.
- W2059187839 cites W1984460944 @default.
- W2059187839 cites W1984987626 @default.
- W2059187839 cites W1987704199 @default.
- W2059187839 cites W1988375781 @default.
- W2059187839 cites W1993315391 @default.
- W2059187839 cites W1996784965 @default.
- W2059187839 cites W2005889545 @default.
- W2059187839 cites W2007608022 @default.
- W2059187839 cites W2008555242 @default.
- W2059187839 cites W2012049188 @default.
- W2059187839 cites W2014416902 @default.
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- W2059187839 cites W2025605247 @default.
- W2059187839 cites W2033284482 @default.
- W2059187839 cites W2038404321 @default.
- W2059187839 cites W2039718244 @default.
- W2059187839 cites W2040479298 @default.
- W2059187839 cites W2041841488 @default.
- W2059187839 cites W2047078918 @default.
- W2059187839 cites W2048273322 @default.
- W2059187839 cites W2051818544 @default.
- W2059187839 cites W2052921451 @default.
- W2059187839 cites W2057463164 @default.
- W2059187839 cites W2063593935 @default.
- W2059187839 cites W2064621113 @default.
- W2059187839 cites W2065532119 @default.
- W2059187839 cites W2066413879 @default.
- W2059187839 cites W2066748993 @default.
- W2059187839 cites W2067163479 @default.
- W2059187839 cites W2068883252 @default.
- W2059187839 cites W2069176827 @default.
- W2059187839 cites W2069747130 @default.
- W2059187839 cites W2074317576 @default.
- W2059187839 cites W2074679317 @default.
- W2059187839 cites W2080614972 @default.
- W2059187839 cites W2085544097 @default.
- W2059187839 cites W2086943348 @default.
- W2059187839 cites W2087786585 @default.
- W2059187839 cites W2088101102 @default.
- W2059187839 cites W2089624112 @default.
- W2059187839 cites W2094204764 @default.
- W2059187839 cites W2096948670 @default.
- W2059187839 cites W2097173606 @default.
- W2059187839 cites W2097178552 @default.
- W2059187839 cites W2099434166 @default.
- W2059187839 cites W2110637060 @default.
- W2059187839 cites W2122874132 @default.
- W2059187839 cites W2123784922 @default.
- W2059187839 cites W2126432370 @default.
- W2059187839 cites W2129042241 @default.
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- W2059187839 cites W2158266834 @default.
- W2059187839 cites W2158771952 @default.
- W2059187839 cites W2160824531 @default.
- W2059187839 cites W2164618384 @default.
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- W2059187839 doi "https://doi.org/10.1093/nar/gks643" @default.
- W2059187839 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3479164" @default.
- W2059187839 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22753106" @default.
- W2059187839 hasPublicationYear "2012" @default.
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