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• W2059201211 abstract "The Na+-Ca2+ exchanger of the cardiac sarcolemma can rapidly transport Ca2+ during excitation-contraction coupling. To begin molecular studies of this transporter, polyclonal antibodies were used to identify a complementary DNA (cDNA) clone encoding the Na+-Ca2+ exchanger protein. The cDNA hybridizes with a 7-kilobase RNA on a Northern blot and has an open reading frame of 970 amino acids. Hydropathy analysis suggests that the protein has multiple transmembrane helices, and a small region of the sequence is similar to that of the Na+- and K+-dependent adenosine triphosphatase. Polyclonal antibodies to a synthetic peptide from the deduced amino acid sequence react with sarcolemmal proteins of 70, 120, and 160 kilodaltons on immunoblots. RNA, synthesized from the cDNA clone, induces expression of Na+-Ca2+ exchange activity when injected into Xenopus oocytes." @default.
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• W2059201211 date "1990-10-26" @default.
• W2059201211 modified "2023-10-16" @default.
• W2059201211 title "Molecular Cloning and Functional Expression of the Cardiac Sarcolemmal Na ⁺ -Ca ²⁺ Exchanger" @default.
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• W2059201211 doi "https://doi.org/10.1126/science.1700476" @default.
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