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- W2059285706 abstract "Thymidylate synthetase has been titrated in mouse liver extracts with [3H]fluorodeoxy-uridine monophosphate using centrifugally eluted gel filtration columns. The method is quick, efficient, and yields a linear dependence upon added extracted protein. The stability of the complex showed a pH maximum near 7.5. Specific binding of the tritiated nucleotide derivative to thymidylate synthetase was shown by denaturation of the complex in sodium dodecyl sulfate and gel electrophoresis of the denatured complex. Centrifugal elution of the sodium dodecyl sulfate-quenched complex from gel filtration columns was used to estimate the kinetics of complex formation at 0 and 12°C." @default.
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- W2059285706 title "A centrifugal column assay for thymidylate synthetase using the active site titrant 5-fluoro-2′-deoxyuridylate" @default.
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- W2059285706 doi "https://doi.org/10.1016/0003-2697(80)90234-1" @default.
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