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- W2059289600 abstract "Mitochondrial Ca2+ handling controls the rate of mitochondrial energy (ATP) production, modulates the spatial and temporal profile of intracellular Ca2+ signaling, regulates mitochondrial ROS generation, and may trigger cell death. Mitochondrial Ca2+ uptake is thought to be mediated by the Ca2+ uniporter (MCU) and other non specific Ca2+ pathways. Recently, a MCU and a non-MCU-type mitochondrial Ca2+ uptake channel were documented in human myocardium. However, existence and electrophysiological properties of calcium channels in murine cardiac mitochondria remain unclear. We isolated cardiac mitoplasts (mitochondria lacking the outer membrane) of mice and performed mitoplast-attached single-channel recordings. By patch-clamping the inner membrane of these cardiac mitochondria we identified two different murine voltage-gated Ca2+ channels, i.e. mCa1 and mCa2. Both channels differed in electrophysiological gating parameters. In the presence of Ca2+ 105 mM in the pipette solution the unitary single-channel amplitude of mCa1 (−1.16±0.03 pA, at −100 mV) was higher than the amplitude of mCa2 (−0.90±0.03 pA). mCa1 showed 3-5 subconductance levels, while mCa2 had only one open state. Moreover, the open probability of mCa1 (0.35±0.05 %) was significantly lower compared to mCa2 (0.97±0.17 %). Both channels could be inhibited by high concentrations of Ruthenium 360 (10 μM), while they were insensitive to blockers of other possibly Ca2+-conducting mitochondrial pores. Single-channel properties suggest that mCa1 underlies the murine MCU. Besides the classical MCU, mitochondrial Ca2+ uptake in mouse heart is mediated via a second voltage-gated Ca2+ channel (mCa2) with distinct properties." @default.
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- W2059289600 date "2012-01-01" @default.
- W2059289600 modified "2023-09-28" @default.
- W2059289600 title "Two Voltage-Gated Calcium Channels Regulate Calcium Uptake in Murine Cardiac Mitochondria" @default.
- W2059289600 doi "https://doi.org/10.1016/j.bpj.2011.11.882" @default.
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