FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2059303964> ?p ?o ?g. }

• W2059303964 endingPage "776" @default.
• W2059303964 startingPage "763" @default.
• W2059303964 abstract "The Sleeping Beauty transposon is a recently developed non-viral vector that can mediate insertion of transgenes into the mammalian genome. Foreign DNA elements that are introduced tend to invoke a host-defense mechanism resulting in epigenetic changes, such as DNA methylation, which may induce transcriptional inactivation of mammalian genes. To assess potential epigenetic modifications associated with Sleeping Beauty transposition, we investigated the DNA methylation pattern of transgenes inserted into the mouse genome as well as genomic regions flanking the insertion sites with bisulfite-mediated genomic sequencing. Transgenic mouse lines were created with two different Sleeping Beauty transposons carrying either the Agouti or eGFP transgene. Our results showed that DNA methylation in the keratin-14 promoter and Agouti transgene were negligible. In addition, two different genomic loci flanking the Agouti insertion site exhibited patterns of DNA methylation similar to wild-type mice. In contrast, high levels of DNA methylation were observed in the eGFP transgene and its ROSA26 promoter. These results indicate that transposition via Sleeping Beauty into the mouse genome may result in a significant level of de novo DNA methylation. This may depend on a number of different factors including the cargo DNA sequence, chromosomal context of the insertion site, and/or host genetic background." @default.
• W2059303964 created "2016-06-24" @default.
• W2059303964 creator A5005626540 @default.
• W2059303964 creator A5007378745 @default.
• W2059303964 creator A5069186777 @default.
• W2059303964 creator A5080573021 @default.
• W2059303964 date "2005-08-01" @default.
• W2059303964 modified "2023-09-24" @default.
• W2059303964 title "DNA methylation of Sleeping Beauty with transposition into the mouse genome" @default.
• W2059303964 cites W1600900798 @default.
• W2059303964 cites W1633991623 @default.
• W2059303964 cites W1967064280 @default.
• W2059303964 cites W1975880129 @default.
• W2059303964 cites W1977087431 @default.
• W2059303964 cites W1992537212 @default.
• W2059303964 cites W1993702378 @default.
• W2059303964 cites W1993881548 @default.
• W2059303964 cites W1999039175 @default.
• W2059303964 cites W2006765654 @default.
• W2059303964 cites W2007814577 @default.
• W2059303964 cites W2031368311 @default.
• W2059303964 cites W2035090622 @default.
• W2059303964 cites W2036300762 @default.
• W2059303964 cites W2040695469 @default.
• W2059303964 cites W2047191657 @default.
• W2059303964 cites W2051974123 @default.
• W2059303964 cites W2052490125 @default.
• W2059303964 cites W2067004457 @default.
• W2059303964 cites W2077166550 @default.
• W2059303964 cites W2083150665 @default.
• W2059303964 cites W2087083968 @default.
• W2059303964 cites W2089903833 @default.
• W2059303964 cites W2094547134 @default.
• W2059303964 cites W2104630023 @default.
• W2059303964 cites W2105801710 @default.
• W2059303964 cites W2118937487 @default.
• W2059303964 cites W2124292811 @default.
• W2059303964 cites W2139122477 @default.
• W2059303964 cites W2148433018 @default.
• W2059303964 cites W2157896232 @default.
• W2059303964 cites W2158374945 @default.
• W2059303964 cites W2161191369 @default.
• W2059303964 cites W2164618384 @default.
• W2059303964 cites W2165993802 @default.
• W2059303964 cites W2168778290 @default.
• W2059303964 cites W2171427593 @default.
• W2059303964 cites W2175849508 @default.
• W2059303964 doi "https://doi.org/10.1111/j.1365-2443.2005.00875.x" @default.
• W2059303964 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16098140" @default.
• W2059303964 hasPublicationYear "2005" @default.
• W2059303964 type Work @default.
• W2059303964 sameAs 2059303964 @default.
• W2059303964 citedByCount "18" @default.
• W2059303964 countsByYear W20593039642012 @default.
• W2059303964 countsByYear W20593039642013 @default.
• W2059303964 countsByYear W20593039642016 @default.
• W2059303964 countsByYear W20593039642018 @default.
• W2059303964 countsByYear W20593039642019 @default.
• W2059303964 crossrefType "journal-article" @default.
• W2059303964 hasAuthorship W2059303964A5005626540 @default.
• W2059303964 hasAuthorship W2059303964A5007378745 @default.
• W2059303964 hasAuthorship W2059303964A5069186777 @default.
• W2059303964 hasAuthorship W2059303964A5080573021 @default.
• W2059303964 hasBestOaLocation W20593039641 @default.
• W2059303964 hasConcept C102230213 @default.
• W2059303964 hasConcept C104317684 @default.
• W2059303964 hasConcept C118083812 @default.
• W2059303964 hasConcept C121912465 @default.
• W2059303964 hasConcept C133288520 @default.
• W2059303964 hasConcept C141231307 @default.
• W2059303964 hasConcept C150194340 @default.
• W2059303964 hasConcept C153911025 @default.
• W2059303964 hasConcept C172510086 @default.
• W2059303964 hasConcept C17757408 @default.
• W2059303964 hasConcept C189235521 @default.
• W2059303964 hasConcept C190727270 @default.
• W2059303964 hasConcept C41091548 @default.
• W2059303964 hasConcept C4918238 @default.
• W2059303964 hasConcept C501734568 @default.
• W2059303964 hasConcept C54355233 @default.
• W2059303964 hasConcept C552990157 @default.
• W2059303964 hasConcept C86803240 @default.
• W2059303964 hasConceptScore W2059303964C102230213 @default.
• W2059303964 hasConceptScore W2059303964C104317684 @default.
• W2059303964 hasConceptScore W2059303964C118083812 @default.
• W2059303964 hasConceptScore W2059303964C121912465 @default.
• W2059303964 hasConceptScore W2059303964C133288520 @default.
• W2059303964 hasConceptScore W2059303964C141231307 @default.
• W2059303964 hasConceptScore W2059303964C150194340 @default.
• W2059303964 hasConceptScore W2059303964C153911025 @default.
• W2059303964 hasConceptScore W2059303964C172510086 @default.
• W2059303964 hasConceptScore W2059303964C17757408 @default.
• W2059303964 hasConceptScore W2059303964C189235521 @default.
• W2059303964 hasConceptScore W2059303964C190727270 @default.
• W2059303964 hasConceptScore W2059303964C41091548 @default.
• W2059303964 hasConceptScore W2059303964C4918238 @default.
• W2059303964 hasConceptScore W2059303964C501734568 @default.
• W2059303964 hasConceptScore W2059303964C54355233 @default.

• next