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• W2059349849 abstract "Abstract The potential of whole‐cell matrix‐assisted laser desorption/ionization time‐of‐flight (MALDI‐TOF) mass spectrometry (MS) for the discovery of microorganisms that produce lantibiotics and/or bacteriocins in the mass range 3–5 kDa is demonstrated. Cells of the antibiotic‐producing model strain Bacillus subtilis ATCC 6633 were grown on Landy agar plates, and prepared with a 9:1 mixture of 2,5‐dihydroxybenzoic acid and 2‐hydroxy‐5‐methoxybenzoic acid (DHBs) as matrix using the bottom‐layer method. Time‐course analyses showed that cells grown for 1–2 days on agar plates gave the best results in terms of intensity of the bacteriocin/lantibiotic signals. Even after storage of matrix‐crystal‐embedded cells for several days high‐resolution spectra (>10 000) could be obtained. To verify the applicability of this technique, different Bacillus wild‐type and gene deletion mutant cells were analyzed. In most of the cases the known antibiotic phenotype of the producer could be corroborated by whole‐cell MALDI‐TOFMS, particularly for the lantibiotics subtilin and ericin. The preparation recipe for whole‐cell analysis by MALDI‐TOF was also highly sensitive for hydrophobic lipopeptide antibiotics like surfactin, mycosubtilin, fengycin, and bacillomycin in the lower mass range (1–1.5 kDa). Copyright © 2008 John Wiley & Sons, Ltd." @default.
• W2059349849 created "2016-06-24" @default.
• W2059349849 creator A5068197565 @default.
• W2059349849 date "2008-03-11" @default.
• W2059349849 modified "2023-10-11" @default.
• W2059349849 title "Whole-cell matrix-assisted laser desorption/ionization mass spectrometry for rapid identification of bacteriocin/lantibiotic-producing bacteria" @default.
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• W2059349849 doi "https://doi.org/10.1002/rcm.3481" @default.
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