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- W2059367635 abstract "<h3>Introduction</h3> Acridine orange has been used for some time as a fluorescent stain for nucleoproteins and for tissue mucopolysaccharides.<sup>6</sup>It has also found use as a vital stain for blood parasites.<sup>8</sup> By accident it was found that the technique of Hicks and Matthaei for the fluorescent staining of mucin<sup>4</sup>would also stain fungi in tissue sections which would then fluoresce under blue-violet light.<sup>7</sup>This finding led to a study of further applications of this method to demonstrate structural details of the fungi themselves and as diagnostic aids in clinical evaluations. <h3>Materials and Methods</h3><h3>Microscope and Filters.—</h3> These studies were done using a Reichert Zetopan microscope with fluorescent attachments. Results were checked periodically with an ordinary binocular microscope and lamp equipped with filters for fluorescent microscopy<sup>7</sup>to insure adaptability to this apparatus. In the latter apparatus a Leitz or Zeiss bluelight fluorescence filter (BG 12, 4 mm." @default.
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- W2059367635 date "1961-02-01" @default.
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- W2059367635 title "Acridine Orange Fluorescent Stain for Fungi" @default.
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- W2059367635 doi "https://doi.org/10.1001/archderm.1961.01580080135015" @default.
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