Matches in SemOpenAlex for { <https://semopenalex.org/work/W2059403208> ?p ?o ?g. }
- W2059403208 endingPage "189" @default.
- W2059403208 startingPage "180" @default.
- W2059403208 abstract "The structure of insulin in amyloid fibrillar form has been recently shown as a well folded conformation using cryoelectron microscopy [Jimenez, J.L., Nettleton, E.J., Bouchard, M., Robinson, C.V., Dobson, C.M., Saibil H.R., 2002. The protofilament structure of insulin amyloid fibrils. Proc. Natl. Acad. Sci. USA. 99 9196–9201.]. Most of the amyloid aggregates elicit maximum toxicity in the protofibrillar (PF) intermediate state. Here, we describe PF intermediates of insulin are made-up monomers with flexible conformers. We also show protofibrils have three-dimensionally extended hydrophobic cavity to bind with 1-anilinonaphthalene-8-sulphonate (ANS) molecules. Energy transfer measurement revealed that ANS dye binding site of PF is within the range of FRET distance of insulin tyrosine residues. Significant proportion of β-sheet, helical, and turn structures in the PF form indicate conformational dynamics in the folded chain of insulin in the PF assembled form. Though the conformational flexibility is noticeably present in the assembly, addition of GdnHCl could completely unfold PF into disordered structure suggesting structural “zipping” in the PF form. We have also shown that helical conformer inducing solvent 2,2,2-trifluoroethanol (TFE) could dissociate the PF aggregate indicating possible involvement of β-sheets in contributing to PF stability." @default.
- W2059403208 created "2016-06-24" @default.
- W2059403208 creator A5031638094 @default.
- W2059403208 creator A5061913983 @default.
- W2059403208 date "2005-05-01" @default.
- W2059403208 modified "2023-10-06" @default.
- W2059403208 title "Spectroscopic studies on native and protofibrillar insulin" @default.
- W2059403208 cites W1494971749 @default.
- W2059403208 cites W1521958691 @default.
- W2059403208 cites W1547655165 @default.
- W2059403208 cites W1969300977 @default.
- W2059403208 cites W1973134139 @default.
- W2059403208 cites W1979509752 @default.
- W2059403208 cites W1984729685 @default.
- W2059403208 cites W1985260080 @default.
- W2059403208 cites W1988089792 @default.
- W2059403208 cites W1991241547 @default.
- W2059403208 cites W1991349307 @default.
- W2059403208 cites W2004463834 @default.
- W2059403208 cites W2008452487 @default.
- W2059403208 cites W2008966525 @default.
- W2059403208 cites W2010462701 @default.
- W2059403208 cites W2016399127 @default.
- W2059403208 cites W2024024256 @default.
- W2059403208 cites W2041365394 @default.
- W2059403208 cites W2051651977 @default.
- W2059403208 cites W2052149626 @default.
- W2059403208 cites W2058398180 @default.
- W2059403208 cites W2068212202 @default.
- W2059403208 cites W2068520497 @default.
- W2059403208 cites W2073516240 @default.
- W2059403208 cites W2075515531 @default.
- W2059403208 cites W2085899859 @default.
- W2059403208 cites W2090235059 @default.
- W2059403208 cites W2090919809 @default.
- W2059403208 cites W2101614182 @default.
- W2059403208 cites W2112226950 @default.
- W2059403208 cites W2117747873 @default.
- W2059403208 cites W2118742600 @default.
- W2059403208 cites W2121963977 @default.
- W2059403208 cites W2125865850 @default.
- W2059403208 cites W2137358718 @default.
- W2059403208 cites W2145538573 @default.
- W2059403208 cites W2145589684 @default.
- W2059403208 cites W2151796887 @default.
- W2059403208 cites W2153439292 @default.
- W2059403208 cites W2154950066 @default.
- W2059403208 cites W2415891402 @default.
- W2059403208 cites W4234193889 @default.
- W2059403208 cites W4235120663 @default.
- W2059403208 cites W4296154147 @default.
- W2059403208 cites W4321429423 @default.
- W2059403208 doi "https://doi.org/10.1016/j.jsb.2005.02.009" @default.
- W2059403208 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15866741" @default.
- W2059403208 hasPublicationYear "2005" @default.
- W2059403208 type Work @default.
- W2059403208 sameAs 2059403208 @default.
- W2059403208 citedByCount "31" @default.
- W2059403208 countsByYear W20594032082012 @default.
- W2059403208 countsByYear W20594032082013 @default.
- W2059403208 countsByYear W20594032082014 @default.
- W2059403208 countsByYear W20594032082015 @default.
- W2059403208 countsByYear W20594032082018 @default.
- W2059403208 countsByYear W20594032082020 @default.
- W2059403208 crossrefType "journal-article" @default.
- W2059403208 hasAuthorship W2059403208A5031638094 @default.
- W2059403208 hasAuthorship W2059403208A5061913983 @default.
- W2059403208 hasConcept C119599485 @default.
- W2059403208 hasConcept C121332964 @default.
- W2059403208 hasConcept C12554922 @default.
- W2059403208 hasConcept C127413603 @default.
- W2059403208 hasConcept C166940927 @default.
- W2059403208 hasConcept C178790620 @default.
- W2059403208 hasConcept C179104552 @default.
- W2059403208 hasConcept C185592680 @default.
- W2059403208 hasConcept C18705241 @default.
- W2059403208 hasConcept C204328495 @default.
- W2059403208 hasConcept C27523624 @default.
- W2059403208 hasConcept C2776545253 @default.
- W2059403208 hasConcept C2777633098 @default.
- W2059403208 hasConcept C32909587 @default.
- W2059403208 hasConcept C47701112 @default.
- W2059403208 hasConcept C521977710 @default.
- W2059403208 hasConcept C55493867 @default.
- W2059403208 hasConcept C62520636 @default.
- W2059403208 hasConcept C71240020 @default.
- W2059403208 hasConcept C8010536 @default.
- W2059403208 hasConcept C86803240 @default.
- W2059403208 hasConcept C91881484 @default.
- W2059403208 hasConcept C96305047 @default.
- W2059403208 hasConceptScore W2059403208C119599485 @default.
- W2059403208 hasConceptScore W2059403208C121332964 @default.
- W2059403208 hasConceptScore W2059403208C12554922 @default.
- W2059403208 hasConceptScore W2059403208C127413603 @default.
- W2059403208 hasConceptScore W2059403208C166940927 @default.
- W2059403208 hasConceptScore W2059403208C178790620 @default.
- W2059403208 hasConceptScore W2059403208C179104552 @default.
- W2059403208 hasConceptScore W2059403208C185592680 @default.