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• W2059457598 startingPage "1941" @default.
• W2059457598 abstract "N-1-Naphthylphthalamic acid (NPA) binding activity is released into the supernatant when plasma membranes are subjected to high-salt treatment, indicating that this activity is peripherally associated with the membrane. Extraction of plasma membrane vesicles with Triton X-100 resulted in retention of NPA binding activity in the detergent-insoluble cytoskeletal pellet. Treatment of this pellet with KI released NPA binding activity, actin, and alpha-tubulin. Dialysis to remove KI led to the repolymerization of cytoskeletal elements and movement of NPA binding activity into an insoluble cytoskeletal pellet. NPA binding activity partitioned into the detergent-insoluble cytoskeletal pellet obtained from both zucchini and maize membranes and was released from these pellets by KI treatment. Treatment of a cytoskeletal pellet with cytochalasin B doubled NPA binding activity in the resulting supernatant. Together, these experiments indicate that NPA binding activity is peripherally associated with the plasma membrane and interacts with the cytoskeleton in vitro." @default.
• W2059457598 created "2016-06-24" @default.
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• W2059457598 creator A5022648068 @default.
• W2059457598 date "1994-12-01" @default.
• W2059457598 modified "2023-09-27" @default.
• W2059457598 title "NPA binding activity is peripheral to the plasma membrane and is associated with the cytoskeleton." @default.
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• W2059457598 doi "https://doi.org/10.1105/tpc.6.12.1941" @default.
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