FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2059489075> ?p ?o ?g. }

• W2059489075 abstract "Abstract The typical archaeal MCM exhibits helicase activity independently in vitro . This study characterizes MCM from the euryarchaeon Picrophilus torridus . While PtMCM hydrolyzes ATP in DNA-independent manner, it displays very poor ability to unwind DNA independently and then too only under acidic conditions. The protein exists stably in complex with PtGINS in whole cell lysates, interacting directly with PtGINS under neutral and acidic conditions. GINS strongly activates MCM helicase activity, but only at low pH. In consonance with this, PtGINS activates PtMCM-mediated ATP hydrolysis only at low pH, with the amount of ATP hydrolyzed during the helicase reaction increasing more than fifty-fold in the presence of GINS. While the stimulation of MCM-mediated helicase activity by GINS has been reported in MCMs from P.furiosus, T.kodakarensis and very recently, T.acidophilum , to the best of our knowledge, this is the first report of an MCM helicase demonstrating DNA unwinding activity only at such acidic pH, across all archaea and eukaryotes. PtGINS may induce/stabilize a conducive conformation of PtMCM under acidic conditions, favouring PtMCM-mediated DNA unwinding coupled to ATP hydrolysis. Our findings underscore the existence of divergent modes of replication regulation among archaea and the importance of investigating replication events in more archaeal organisms." @default.
• W2059489075 created "2016-06-24" @default.
• W2059489075 creator A5008112972 @default.
• W2059489075 creator A5028315481 @default.
• W2059489075 creator A5040223755 @default.
• W2059489075 date "2015-03-12" @default.
• W2059489075 modified "2023-10-14" @default.
• W2059489075 title "Characterization of the MCM homohexamer from the thermoacidophilic euryarchaeon Picrophilus torridus" @default.
• W2059489075 cites W1959624233 @default.
• W2059489075 cites W1969224299 @default.
• W2059489075 cites W1971754542 @default.
• W2059489075 cites W1972926466 @default.
• W2059489075 cites W1981075146 @default.
• W2059489075 cites W1986455228 @default.
• W2059489075 cites W1990520992 @default.
• W2059489075 cites W1993424636 @default.
• W2059489075 cites W1996221501 @default.
• W2059489075 cites W1997827029 @default.
• W2059489075 cites W2001879903 @default.
• W2059489075 cites W2004763144 @default.
• W2059489075 cites W2013833462 @default.
• W2059489075 cites W2015012409 @default.
• W2059489075 cites W2015901264 @default.
• W2059489075 cites W2032554048 @default.
• W2059489075 cites W2033503236 @default.
• W2059489075 cites W2034773869 @default.
• W2059489075 cites W2034902378 @default.
• W2059489075 cites W2037491280 @default.
• W2059489075 cites W2049779938 @default.
• W2059489075 cites W2050009050 @default.
• W2059489075 cites W2050241400 @default.
• W2059489075 cites W2050851430 @default.
• W2059489075 cites W2056003750 @default.
• W2059489075 cites W2059010765 @default.
• W2059489075 cites W2059806137 @default.
• W2059489075 cites W2060919343 @default.
• W2059489075 cites W2066360834 @default.
• W2059489075 cites W2068578100 @default.
• W2059489075 cites W2069607114 @default.
• W2059489075 cites W2069836382 @default.
• W2059489075 cites W2070336258 @default.
• W2059489075 cites W2078740297 @default.
• W2059489075 cites W2086104176 @default.
• W2059489075 cites W2091369957 @default.
• W2059489075 cites W2093095333 @default.
• W2059489075 cites W2097033734 @default.
• W2059489075 cites W2100240627 @default.
• W2059489075 cites W2126843113 @default.
• W2059489075 cites W2126909443 @default.
• W2059489075 cites W2134156532 @default.
• W2059489075 cites W2138643693 @default.
• W2059489075 cites W2146677747 @default.
• W2059489075 cites W2150270428 @default.
• W2059489075 cites W2156933152 @default.
• W2059489075 cites W2160131663 @default.
• W2059489075 cites W2163882140 @default.
• W2059489075 cites W2168329140 @default.
• W2059489075 cites W3198823065 @default.
• W2059489075 cites W4247812387 @default.
• W2059489075 doi "https://doi.org/10.1038/srep09057" @default.
• W2059489075 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4356968" @default.
• W2059489075 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25762096" @default.
• W2059489075 hasPublicationYear "2015" @default.
• W2059489075 type Work @default.
• W2059489075 sameAs 2059489075 @default.
• W2059489075 citedByCount "8" @default.
• W2059489075 countsByYear W20594890752016 @default.
• W2059489075 countsByYear W20594890752017 @default.
• W2059489075 countsByYear W20594890752018 @default.
• W2059489075 countsByYear W20594890752019 @default.
• W2059489075 crossrefType "journal-article" @default.
• W2059489075 hasAuthorship W2059489075A5008112972 @default.
• W2059489075 hasAuthorship W2059489075A5028315481 @default.
• W2059489075 hasAuthorship W2059489075A5040223755 @default.
• W2059489075 hasBestOaLocation W20594890751 @default.
• W2059489075 hasConcept C104317684 @default.
• W2059489075 hasConcept C116107829 @default.
• W2059489075 hasConcept C12554922 @default.
• W2059489075 hasConcept C141315368 @default.
• W2059489075 hasConcept C156719811 @default.
• W2059489075 hasConcept C161223559 @default.
• W2059489075 hasConcept C181199279 @default.
• W2059489075 hasConcept C185592680 @default.
• W2059489075 hasConcept C194830204 @default.
• W2059489075 hasConcept C23265538 @default.
• W2059489075 hasConcept C2776943354 @default.
• W2059489075 hasConcept C550995028 @default.
• W2059489075 hasConcept C552990157 @default.
• W2059489075 hasConcept C55493867 @default.
• W2059489075 hasConcept C67705224 @default.
• W2059489075 hasConcept C73573662 @default.
• W2059489075 hasConcept C86803240 @default.
• W2059489075 hasConcept C94412978 @default.
• W2059489075 hasConcept C95444343 @default.
• W2059489075 hasConceptScore W2059489075C104317684 @default.
• W2059489075 hasConceptScore W2059489075C116107829 @default.
• W2059489075 hasConceptScore W2059489075C12554922 @default.
• W2059489075 hasConceptScore W2059489075C141315368 @default.
• W2059489075 hasConceptScore W2059489075C156719811 @default.
• W2059489075 hasConceptScore W2059489075C161223559 @default.

• next