FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2059496978> ?p ?o ?g. }

• W2059496978 endingPage "158" @default.
• W2059496978 startingPage "151" @default.
• W2059496978 abstract "Summary Immune responses in the Australian common brushtail possum ( Trichosurus vulpecula ) and in particular the role of cytokines are poorly understood. We have undertaken to isolate cytokine genes using reverse transcriptase‐polymerase chain reaction (RT‐PCR) and in this study describe the molecular cloning of TNF‐α. Primers were designed from consensus sequences at the N ‐terminus end of eutherian mammalian TNF‐α and the possum cDNA, derived from spleen RNA, identified by RT‐PCR. The complete cDNA encoding possum TNF‐α was amplified from lymphocyte RNA by 5′ and 3′ rapid amplification of cDNA ends (RACE). The nucleotide sequence of the protein coding region of this cDNA shared 66‐69% identity with other mammalian TNF‐α genes. The predicted protein of 233 amino acids shared 56‐58% identity with eutherian mammalian TNF‐α. Possum TNF‐α was expressed in both Saceharomyces cerevisiae and Escherichia coli by constructing expression plasmid derivatives of the vectors pYES2 and pGEX‐2T respectively. Cell extracts prepared from transformants and the purified GST/TNF‐α fusion protein exhibited cytotoxic activity on the TNF‐α‐sensitive murine fibroblast L929 cells and stimulated proliferation of possum thymocyte cells. The induction of possum TNF‐α mRNA in alveolar macrophages was analysed by RT‐PCR using possumspecific TNF‐α primers. Macrophages cultured in the presence of LPS showed enhanced transcription of TNF‐α mRNA. This is the first report of the cloning and sequence analysis of the cDNA encoding a marsupial cytokine gene." @default.
• W2059496978 created "2016-06-24" @default.
• W2059496978 creator A5000575764 @default.
• W2059496978 creator A5061243499 @default.
• W2059496978 creator A5090792712 @default.
• W2059496978 date "1996-04-01" @default.
• W2059496978 modified "2023-10-16" @default.
• W2059496978 title "Molecular cloning and characterization of tumour necrosis factor alpha (TNF‐α) from the Australian common brushtail possum, <i>Trichosurus vulpecula</i>" @default.
• W2059496978 cites W1503094954 @default.
• W2059496978 cites W1973782344 @default.
• W2059496978 cites W1990590909 @default.
• W2059496978 cites W1992071813 @default.
• W2059496978 cites W1992981549 @default.
• W2059496978 cites W2001426244 @default.
• W2059496978 cites W2012739094 @default.
• W2059496978 cites W2012886159 @default.
• W2059496978 cites W2020114653 @default.
• W2059496978 cites W2034040209 @default.
• W2059496978 cites W2034971028 @default.
• W2059496978 cites W2046621497 @default.
• W2059496978 cites W2055090334 @default.
• W2059496978 cites W2057473278 @default.
• W2059496978 cites W2070297702 @default.
• W2059496978 cites W2078684076 @default.
• W2059496978 cites W2080532845 @default.
• W2059496978 cites W2085486418 @default.
• W2059496978 cites W2087405688 @default.
• W2059496978 cites W2108388593 @default.
• W2059496978 cites W2319684336 @default.
• W2059496978 doi "https://doi.org/10.1038/icb.1996.20" @default.
• W2059496978 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8724002" @default.
• W2059496978 hasPublicationYear "1996" @default.
• W2059496978 type Work @default.
• W2059496978 sameAs 2059496978 @default.
• W2059496978 citedByCount "29" @default.
• W2059496978 countsByYear W20594969782012 @default.
• W2059496978 countsByYear W20594969782013 @default.
• W2059496978 countsByYear W20594969782014 @default.
• W2059496978 countsByYear W20594969782015 @default.
• W2059496978 countsByYear W20594969782016 @default.
• W2059496978 countsByYear W20594969782018 @default.
• W2059496978 crossrefType "journal-article" @default.
• W2059496978 hasAuthorship W2059496978A5000575764 @default.
• W2059496978 hasAuthorship W2059496978A5061243499 @default.
• W2059496978 hasAuthorship W2059496978A5090792712 @default.
• W2059496978 hasConcept C104317684 @default.
• W2059496978 hasConcept C105580179 @default.
• W2059496978 hasConcept C121050878 @default.
• W2059496978 hasConcept C151730666 @default.
• W2059496978 hasConcept C153911025 @default.
• W2059496978 hasConcept C156719811 @default.
• W2059496978 hasConcept C17991360 @default.
• W2059496978 hasConcept C187882448 @default.
• W2059496978 hasConcept C19924922 @default.
• W2059496978 hasConcept C199360897 @default.
• W2059496978 hasConcept C203014093 @default.
• W2059496978 hasConcept C2777014246 @default.
• W2059496978 hasConcept C2777042810 @default.
• W2059496978 hasConcept C41008148 @default.
• W2059496978 hasConcept C4692481 @default.
• W2059496978 hasConcept C50724178 @default.
• W2059496978 hasConcept C54355233 @default.
• W2059496978 hasConcept C67705224 @default.
• W2059496978 hasConcept C86803240 @default.
• W2059496978 hasConceptScore W2059496978C104317684 @default.
• W2059496978 hasConceptScore W2059496978C105580179 @default.
• W2059496978 hasConceptScore W2059496978C121050878 @default.
• W2059496978 hasConceptScore W2059496978C151730666 @default.
• W2059496978 hasConceptScore W2059496978C153911025 @default.
• W2059496978 hasConceptScore W2059496978C156719811 @default.
• W2059496978 hasConceptScore W2059496978C17991360 @default.
• W2059496978 hasConceptScore W2059496978C187882448 @default.
• W2059496978 hasConceptScore W2059496978C19924922 @default.
• W2059496978 hasConceptScore W2059496978C199360897 @default.
• W2059496978 hasConceptScore W2059496978C203014093 @default.
• W2059496978 hasConceptScore W2059496978C2777014246 @default.
• W2059496978 hasConceptScore W2059496978C2777042810 @default.
• W2059496978 hasConceptScore W2059496978C41008148 @default.
• W2059496978 hasConceptScore W2059496978C4692481 @default.
• W2059496978 hasConceptScore W2059496978C50724178 @default.
• W2059496978 hasConceptScore W2059496978C54355233 @default.
• W2059496978 hasConceptScore W2059496978C67705224 @default.
• W2059496978 hasConceptScore W2059496978C86803240 @default.
• W2059496978 hasIssue "2" @default.
• W2059496978 hasLocation W20594969781 @default.
• W2059496978 hasLocation W20594969782 @default.
• W2059496978 hasOpenAccess W2059496978 @default.
• W2059496978 hasPrimaryLocation W20594969781 @default.
• W2059496978 hasRelatedWork W175443584 @default.
• W2059496978 hasRelatedWork W2005559168 @default.
• W2059496978 hasRelatedWork W2059496978 @default.
• W2059496978 hasRelatedWork W2121396784 @default.
• W2059496978 hasRelatedWork W2403784467 @default.
• W2059496978 hasRelatedWork W2416218981 @default.
• W2059496978 hasRelatedWork W2943343189 @default.
• W2059496978 hasRelatedWork W3031220128 @default.
• W2059496978 hasRelatedWork W4240552046 @default.
• W2059496978 hasRelatedWork W4303983510 @default.

• next