FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2059565941> ?p ?o ?g. }

• W2059565941 endingPage "861" @default.
• W2059565941 startingPage "852" @default.
• W2059565941 abstract "Much controversy has arisen on the nature and sources of stem cells in the adult human retina. Whilst ciliary epithelium has been thought to constitute a source of neural stem cells, a population of Müller glia in the neural retina has also been shown to exhibit neurogenic characteristics. This study aimed to compare the neurogenic and proliferative abilities between these two major cell populations. It also examined whether differences exist between the pigmented and non-pigmented ciliary epithelium (CE) from the adult human eye. On this basis, Müller glia with stem cell characteristics and pigmented and non-pigmented CE were isolated from human neural retina and ciliary epithelium respectively. Expression of glial, epithelial and neural progenitor markers was examined in these cells following culture under adherent and non-adherent conditions and treatments to induce neural differentiation. Unlike pigmented CE which did not proliferate, non-pigmented CE cells exhibited limited proliferation in vitro, unless epidermal growth factor (EGF) was present in the culture medium to prolong their survival. In contrast, Müller glial stem cells (MSC) cultured as adherent monolayers reached confluence within a few weeks and continued to proliferative indefinitely in the absence of EGF. Both MSC and non-pigmented CE expressed markers of neural progenitors, including SOX2, PAX6, CHX10 and NOTCH. Nestin, a neural stem cell marker, was only expressed by MSC. Non-pigmented CE displayed epithelial morphology, limited photoreceptor gene expression and stained strongly for pigmented epithelial markers upon culture with neural differentiation factors. In contrast, MSC adopted neural morphology and expressed markers of retinal ganglion cells and photoreceptors when cultured under similar conditions. This study provides the first demonstration that pigmented CE possess different proliferative abilities from non-pigmented CE. It also showed that although non-pigmented CE express genes of retinal progenitors, they do not differentiate into neurons in vitro, as that seen with Müller glia that proliferate indefinitely in vitro and that acquire markers of retinal neurons in culture under neural differentiation protocols. From these observations it is possible to suggest that Müller glia that express markers of neural progenitors and become spontaneously immortalized in vitro constitute a potential source of retinal neurons for transplantation studies and fulfil the characteristics of true stem cells due to their proliferative and neurogenic ability." @default.
• W2059565941 created "2016-06-24" @default.
• W2059565941 creator A5007347867 @default.
• W2059565941 creator A5046032443 @default.
• W2059565941 creator A5065787639 @default.
• W2059565941 creator A5072417653 @default.
• W2059565941 creator A5078606687 @default.
• W2059565941 date "2011-12-01" @default.
• W2059565941 modified "2023-10-04" @default.
• W2059565941 title "Differences between the neurogenic and proliferative abilities of Müller glia with stem cell characteristics and the ciliary epithelium from the adult human eye" @default.
• W2059565941 cites W1964163541 @default.
• W2059565941 cites W1964728628 @default.
• W2059565941 cites W1967980430 @default.
• W2059565941 cites W1978371504 @default.
• W2059565941 cites W1996130915 @default.
• W2059565941 cites W2000718430 @default.
• W2059565941 cites W2008294065 @default.
• W2059565941 cites W2013433258 @default.
• W2059565941 cites W2016552437 @default.
• W2059565941 cites W2030136275 @default.
• W2059565941 cites W2034977638 @default.
• W2059565941 cites W2037390777 @default.
• W2059565941 cites W2046793862 @default.
• W2059565941 cites W2058963589 @default.
• W2059565941 cites W2062746860 @default.
• W2059565941 cites W2063504478 @default.
• W2059565941 cites W2076188667 @default.
• W2059565941 cites W2084582407 @default.
• W2059565941 cites W2087357183 @default.
• W2059565941 cites W2097832718 @default.
• W2059565941 cites W2099636023 @default.
• W2059565941 cites W2101777616 @default.
• W2059565941 cites W2110385326 @default.
• W2059565941 cites W2114268866 @default.
• W2059565941 cites W2116718309 @default.
• W2059565941 cites W2116869556 @default.
• W2059565941 cites W2131416478 @default.
• W2059565941 cites W2146839421 @default.
• W2059565941 cites W2157381025 @default.
• W2059565941 cites W2162463187 @default.
• W2059565941 cites W2165713377 @default.
• W2059565941 doi "https://doi.org/10.1016/j.exer.2011.09.015" @default.
• W2059565941 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3268355" @default.
• W2059565941 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21989110" @default.
• W2059565941 hasPublicationYear "2011" @default.
• W2059565941 type Work @default.
• W2059565941 sameAs 2059565941 @default.
• W2059565941 citedByCount "56" @default.
• W2059565941 countsByYear W20595659412012 @default.
• W2059565941 countsByYear W20595659412013 @default.
• W2059565941 countsByYear W20595659412014 @default.
• W2059565941 countsByYear W20595659412015 @default.
• W2059565941 countsByYear W20595659412016 @default.
• W2059565941 countsByYear W20595659412017 @default.
• W2059565941 countsByYear W20595659412018 @default.
• W2059565941 countsByYear W20595659412019 @default.
• W2059565941 countsByYear W20595659412020 @default.
• W2059565941 countsByYear W20595659412021 @default.
• W2059565941 countsByYear W20595659412022 @default.
• W2059565941 countsByYear W20595659412023 @default.
• W2059565941 crossrefType "journal-article" @default.
• W2059565941 hasAuthorship W2059565941A5007347867 @default.
• W2059565941 hasAuthorship W2059565941A5046032443 @default.
• W2059565941 hasAuthorship W2059565941A5065787639 @default.
• W2059565941 hasAuthorship W2059565941A5072417653 @default.
• W2059565941 hasAuthorship W2059565941A5078606687 @default.
• W2059565941 hasBestOaLocation W20595659411 @default.
• W2059565941 hasConcept C104317684 @default.
• W2059565941 hasConcept C136302470 @default.
• W2059565941 hasConcept C136834591 @default.
• W2059565941 hasConcept C142724271 @default.
• W2059565941 hasConcept C145103041 @default.
• W2059565941 hasConcept C148738053 @default.
• W2059565941 hasConcept C162746124 @default.
• W2059565941 hasConcept C169760540 @default.
• W2059565941 hasConcept C200707615 @default.
• W2059565941 hasConcept C201750760 @default.
• W2059565941 hasConcept C20875687 @default.
• W2059565941 hasConcept C2777093970 @default.
• W2059565941 hasConcept C2777102633 @default.
• W2059565941 hasConcept C2777933648 @default.
• W2059565941 hasConcept C28328180 @default.
• W2059565941 hasConcept C51816534 @default.
• W2059565941 hasConcept C54355233 @default.
• W2059565941 hasConcept C71924100 @default.
• W2059565941 hasConcept C86339819 @default.
• W2059565941 hasConcept C86803240 @default.
• W2059565941 hasConcept C95444343 @default.
• W2059565941 hasConceptScore W2059565941C104317684 @default.
• W2059565941 hasConceptScore W2059565941C136302470 @default.
• W2059565941 hasConceptScore W2059565941C136834591 @default.
• W2059565941 hasConceptScore W2059565941C142724271 @default.
• W2059565941 hasConceptScore W2059565941C145103041 @default.
• W2059565941 hasConceptScore W2059565941C148738053 @default.
• W2059565941 hasConceptScore W2059565941C162746124 @default.
• W2059565941 hasConceptScore W2059565941C169760540 @default.
• W2059565941 hasConceptScore W2059565941C200707615 @default.
• W2059565941 hasConceptScore W2059565941C201750760 @default.

• next