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- W2059575462 abstract "Abstract The glycoprotein nicastrin (NCT) is an essential component of the γ‐secretase complex, a high molecular weight complex which also contains the presenilin proteins, Aph‐1 and Pen‐2. The γ‐secretase complex is not only involved in APP processing but also in the processing of an increasing number of other type I integral membrane proteins. As the largest subunit of the γ‐secretase complex, NCT plays a crucial role in its activation. Considerable information exists on the distribution, structure and function of NCT; however, little is known of its proteolysis. The present study is aimed at exploring the molecular mechanism of NCT degradation. We found that either proteasomal or lysosomal inhibition can significantly increase the levels of both endogenous and exogenous NCT in various cell lines, and the effect of these inhibitions on NCT was time‐ and dose‐dependent. Immunofluorescent microscopic analysis revealed that NCT accumulates in the ER and Golgi apparatus after proteasomal inhibition, while lysosomal inhibition leads to the accumulation of NCT in the lysosomal apparatus. Co‐immunoprecipitation can pull down both NCT and ubiquitin. Taken together, our results demonstrate that NCT degradation involves both the proteasome and the lysosome." @default.
- W2059575462 created "2016-06-24" @default.
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- W2059575462 creator A5071165085 @default.
- W2059575462 creator A5076881573 @default.
- W2059575462 date "2007-01-22" @default.
- W2059575462 modified "2023-10-11" @default.
- W2059575462 title "Degradation of nicastrin involves both proteasome and lysosome" @default.
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- W2059575462 doi "https://doi.org/10.1111/j.1471-4159.2007.04449.x" @default.
- W2059575462 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17326768" @default.
- W2059575462 hasPublicationYear "2007" @default.
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