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• W2059584015 abstract "E3 ubiquitin ligases catalyze the conjugation of ubiquitin onto proteins, which acts as a signal for targeting proteins for degradation by the proteasome. Hrd1 is an endoplasmic reticulum (ER) membrane-spanning E3 with its catalytic active RING finger facing the cytosol. We speculated that this topology might allow Hrd1 to ubiquitinate misfolded proteins in the cytosol. We tested this idea by using polyglutamine (polyQ)-containing huntingtin (htt) protein as a model substrate. We found that the protein levels of Hrd1 were increased in cells overexpressing the N-terminal fragment of htt containig an expanded polyQ tract (httN). Forced expression of Hrd1 enhanced the degradation of httN in a RING finger-dependent manner, whereas silencing of endogenous Hrd1 expression by RNA interference stabilized httN. Degradation of httN was found to be p97/VCP-dependent, but independent of Ufd1 and Npl4, all of which are thought to form a complex with Hrd1 during ER-associated degradation. Consistent with its role as an E3 for httN, we demonstrate that Hrd1 interacts with and ubiquitinates httN. Subcellular fractionation and confocal microscopy revealed that Hrd1recruits HttN to the ER and co-localizes with juxtanuclear aggregates of httN in cells. Interaction of Hrd1 with httN was found to be independent of the length of the polyglutamine tract. However, httN with expanded polyglutamine tracts appeared to be a preferred substrate for Hrd1. Functionally, we found that Hrd1 protects cells against the httN-induced cell death. These results suggest that Hrd1 is a novel htt-interacting protein that can target pathogenic httN for degradation and is able to protect cells against httN-induced cell death." @default.
• W2059584015 created "2016-06-24" @default.
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• W2059584015 date "2007-02-01" @default.
• W2059584015 modified "2023-10-13" @default.
• W2059584015 title "Ubiquitin ligase Hrd1 enhances the degradation and suppresses the toxicity of polyglutamine-expanded huntingtin" @default.
• W2059584015 cites W1504496748 @default.
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• W2059584015 cites W1708445842 @default.
• W2059584015 cites W1940885739 @default.
• W2059584015 cites W1973897761 @default.
• W2059584015 cites W1977733590 @default.
• W2059584015 cites W1980420611 @default.
• W2059584015 cites W1986627314 @default.
• W2059584015 cites W1987927755 @default.
• W2059584015 cites W1988146888 @default.
• W2059584015 cites W1988740579 @default.
• W2059584015 cites W1993050097 @default.
• W2059584015 cites W1999124126 @default.
• W2059584015 cites W2007422965 @default.
• W2059584015 cites W2011368229 @default.
• W2059584015 cites W2013644117 @default.
• W2059584015 cites W2014497390 @default.
• W2059584015 cites W2018462246 @default.
• W2059584015 cites W2021242983 @default.
• W2059584015 cites W2022376508 @default.
• W2059584015 cites W2023053159 @default.
• W2059584015 cites W2023662773 @default.
• W2059584015 cites W2023680254 @default.
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• W2059584015 cites W2030560450 @default.
• W2059584015 cites W2033355252 @default.
• W2059584015 cites W2040251321 @default.
• W2059584015 cites W2042036635 @default.
• W2059584015 cites W2042716629 @default.
• W2059584015 cites W2045151558 @default.
• W2059584015 cites W2045504804 @default.
• W2059584015 cites W2050996044 @default.
• W2059584015 cites W2054756404 @default.
• W2059584015 cites W2057668553 @default.
• W2059584015 cites W2060148506 @default.
• W2059584015 cites W2061151435 @default.
• W2059584015 cites W2061801364 @default.
• W2059584015 cites W2064444660 @default.
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• W2059584015 cites W2071358321 @default.
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• W2059584015 cites W2141928432 @default.
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• W2059584015 doi "https://doi.org/10.1016/j.yexcr.2006.10.031" @default.
• W2059584015 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17141218" @default.
• W2059584015 hasPublicationYear "2007" @default.
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