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- W2059640959 abstract "Microfluidic devices with the versatility of precisely controlling the immediate micro-environment of a single cell or a small population of cells have resulted in a paradigm shift in studying cellular development, local sub-cellular cell signaling, and ligand-activated dynamic cellular responses. In this paper, we report the development of multi-layered PDMS microfluidic devices that are dynamically tunable to provide on-demand, different spatio-temporally regulated micro-environments to in-vitro cultures of primary cells, like neurons and myotubes. The cell culture chamber was made in PDMS by exclusion-molding from a SU-8 master. The pneumatic actuator channel was molded separately, aligned and plasma-bonded on top of the cell-culture chamber. The roof of the cell-chamber contained elastomeric actuators designed to generate a dynamically-addressable, isolated compartment within the chamber. The separation of fluidic micro-domains was characterized with dyes. This tunable device can be used to (a) co-culture spatially separated cell populations, (b) dynamically separate axonal and somal compartments of a neuron, and (c) focally incubate sub-cellular regions of myotubes with extra-cellular biomolecules and ligands. We demonstrate time-lapse imaging and simulated models using the finite-element method, to show the dynamic tuning of the microfluidic channels, in the absence as well as in the presence of cells." @default.
- W2059640959 created "2016-06-24" @default.
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- W2059640959 date "2009-02-01" @default.
- W2059640959 modified "2023-09-28" @default.
- W2059640959 title "Tunable Microfluidic Devices for Dynamically Controlling Sub-Cellular Environments" @default.
- W2059640959 doi "https://doi.org/10.1016/j.bpj.2008.12.2951" @default.
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